A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

0:56

Creating a New CryoSPARC v3 Project and Importing Data

1:34

CryoSPARC v3: Movie Alignment and Contrast Transfer Function (CTF) Estimation

2:52

CryoSPARC v3: Manual and Template-Based Particle Picking

4:21

CryoSPARC v3: 2D Classification

4:52

CryoSPARC v3: Ab-initio Reconstruction and Homogeneous Refinement

5:31

Exporting Particle Coordinates from CryoSPARC v3 and Importing Them to RELION-3 Using PyEM

5:56

RELION-3: Particle Extraction and 2D Classification, 3D Refinement, Mask Creation, and Post-Processing

7:41

RELION-3: Polishing Training, Particle Polishing, CTF, and Per-Particle Refinements

9:15

Transferring RELION-3 Particle Coordinates and 3D Map to Scipion 3

10:05

Scipion 3: High-Resolution Refinement

11:21

Results: Using cryoSPARC v3, RELION-3, and Scipion 3 to Obtain a High-Resolution 3D Reconstruction of the Adeno-Associated Virus

12:52

Conclusion