This work was supported by a grant from the Heart and Stroke Foundation of Canada and by The Morris and Bella Fainman Family Foundation. The authors would like to thank Veronique Michaud for her technical help with the VEVO770 ultrasound imaging system.

All procedures were approved by the institutional Animal Care Committee of McGill University Montr&#233;al, QC, Canada. All the equipment required is listed in Table I.
1. Murine (C57BL/6J) IVC Stasis Protocol
<ol>
	<li>Surgery preparation 
	NOTE: This is a survival surgery and therefore, proper aseptic technique needs to be followed at all times. This includes making sure all materials are at hand, cleaning and sterilizing instruments before and between use, and designating a sterile area on the working surface for all sterile material.
	<ol>
		<li>Sterilize all surgical instruments&#160;and materials by autoclave. A glass bead sterilizer is sufficient to sterilize instrument tips between animals, if more than one procedure is being done at one time.</li>
		<li>Anesthetize 8 - 10 week old C57Bl/6 male mice with a mixture of 100% oxygen and 2.5% isoflurane. 
		NOTE: Adjust the flow of oxygen and isoflurane as necessary.</li>
		<li>Confirm anesthetization by pinching the rear paw of the animal between the toes with forceps. No response from the pinch indicates the animal is anesthetized.</li>
		<li>Administer slow release analgesic (buprenorphine) via subcutaneous injection. Give mice a dose of 1 mg of analgesic per 1 kg of body weight (1 mg/kg).</li>
		<li>Place eye ointment on both eyes of the animal to ensure no corneal desiccation for the duration of the procedure.</li>
		<li>Administer 0.2 - 0.5 mL of isotonic fluids per 10 g of body weight subcutaneously.</li>
		<li>Fix the animal in place to the surgery table using surgical tape. Place the animal in a supine position to expose the abdomen. Perform surgery on a heating pad to prevent hypothermia.</li>
		<li>Remove hair from the abdomen of the mouse by application of a hair removal cream for 1 - 2 min. Wipe the abdominal area clean with gauze and distilled water, if necessary.</li>
		<li>Alternatively, remove the hair from the abdomen by shaving with a small electric razor.</li>
		<li>Sterilize the abdomen with&#160;Baxedin (Chlorhexidine Gluconate BP Solution)&#160;&#160;prior to making any incisions. Apply ethanol lightly with gauze to avoid excessive heat loss caused by the evaporation of the alcohol.</li>
	</ol>
	</li>
	<li>Exposure of the Inferior Vena Cava (IVC)
	<ol>
		<li>Using surgical scissors, perform a laparotomy to open the abdominal cavity.
		<ol>
			<li>Lift skin with forceps at the lower abdomen and make a vertical incision parallel to either side of the linea alba. Make the first incision to the left or right of the linea alba, depending on the handedness of the operator. The incision is made away from the midline to assist in ultrasound imaging later on.</li>
			<li>Make a second, horizontal incision at the top of the abdomen. 
			NOTE: Upon penetration into the abdomen, the musculature should be &#8220;tented&#8221; and then penetrated with surgical scissors to prevent any damage to the abdominal organs.</li>
			<li>Repeat the vertical and horizontal incisions on the abdominal muscle layer of the animal.&#160;Be sure to tent the musculature during penetration into the abdomen so as not to damage the organs beneath. 
			Caution: Lift skin and muscle away from intestines and other internal organs when making incisions so as not to puncture them.</li>
		</ol>
		</li>
		<li>Fold the skin and muscle away from the incision to expose the abdominal cavity.</li>
		<li>Apply gauze, moistened with isotonic solution, to the sides of the wound opening and externalize the intestines. Apply pressure to both sides of the abdomen to assist the externalization of the intestines. Use gentle movements with a cotton tip applicator to move the intestines. Soak gauze in isotonic solution and place it over the externalized intestines.&#160;
		<ol>
			<li>Gauze should be pre-moistened prior to placement on tissue.</li>
		</ol>
		</li>
		<li>Move aside any peritoneal fat, and expose the IVC between the renal and iliac veins.</li>
		<li>Make an initial identification of obvious side branches. A side branch will appear as smaller, but significant vein that branches away from the IVC, between the renal and iliac veins. Vasculature can vary greatly between mice. Mice may have side branches present on one or both sides of the IVC.</li>
	</ol>
	</li>
	<li>Ligation of side branches 
	NOTE: If side branches are present, the following procedure is followed for each one. If not present, proceed to step 4: Initial suture placement under the IVC. The ligation site of the IVC will be immediately distal to the left renal vein, regardless of the presence of side branches.
	<ol>
		<li>Perform blunt dissection on each side of the side branch. Blunt dissection is the process of repeatedly opening blunt forceps with gentle pressure, so as to break through fascia without damaging surrounding vessels. 
		Caution: Avoid puncturing or damaging the vein.</li>
		<li>Lift the branch and pass a section of 6-0 silk suture underneath the vein. Always take care when lifting or moving vessels. It is best to grab the fat around the vessels, otherwise there is a risk of damaging the vessel.</li>
		<li>Using suture forceps, make a surgical ligation around the side branch using standard surgical knot tying technique. Full ligation is done to ensure 100% occlusion of the vein. Use at least three tying throws to ensure the ligation is secure.</li>
		<li>Repeat step 1.3 for any remaining side branches.</li>
	</ol>
	</li>
	<li>Dissection of the abdominal aorta from the IVC
	<ol>
		<li>Perform blunt dissection around the IVC immediately distal from the left renal vein. The abdominal aorta is attached to the IVC via fascia, therefore perform initial blunt dissection around both the IVC and abdominal aorta. 
		Caution: Avoid puncturing or damaging the vein. Do not rupture or puncture either vessel. This is the most critical part of the procedure, with the highest risk for vessel damage.</li>
		<li>Continue to apply gentle pressure via blunt dissection until a clear window is made between the aorta and IVC.</li>
	</ol>
	</li>
	<li>Suture placement and IVC ligation
	<ol>
		<li>Pass a section of 6-0 silk suture underneath the IVC and abdominal aorta.</li>
		<li>Locate the suture through the window created between the IVC and aorta. Use forceps to pull the suture and thread it between the abdominal aorta and IVC. Thread the suture through carefully, trying not to pull/puncture the IVC and abdominal aorta.</li>
		<li>Make a surgical ligation around the IVC with suture forceps, ensuring a total occlusion of the vein. Again, make the ligation immediately distal from the left renal vein.</li>
		<li>Make three suture throws to secure the ligation. 
		NOTE: It is also possible to pass the suture underneath the IVC and aorta prior to separating the two as an alternative option.&#160;Alternatively, 7-0 Prolene sutures can be used for ligation of the side branches and the IVC.</li>
	</ol>
	</li>
	<li>Wound closure and post-operative care
	<ol>
		<li>Verify that the abdominal aorta has been left uninterrupted and that all side branches have been ligated. The IVC will appear dilated distal from the ligation site and no blood flow will be visible.</li>
		<li>Place all peritoneal fat and the intestines back in the abdominal cavity.</li>
		<li>Close the wound using suture forceps and 6-0 silk suture. Use a running suture (simple continuous) and ensure that the suture will not be too tight. A tight suture will rupture when the animal wakes up and moves around its cage.
		<ol>
			<li>Alternatively, use PDS, Ethilon, Vicryl, Dexon, or stainless steel clips for wound closure.</li>
		</ol>
		</li>
		<li>Suture the layer of abdominal muscle first using appropriate suture tying technique.</li>
		<li>Repeat suture technique for the skin and verify wound closure is sufficient. 
		NOTE: If using the same suture needle and thread for multiple animals, sterilize both in 70% ethanol prior to each use.</li>
		<li>Remove the animal from anesthetic gas and place them in a 34 &#176;C incubator for at least 30 minutes.</li>
		<li>Again, administer 0.2 - 0.5 mL of isotonic fluids per 10 g of body weight subcutaneously. Fluids can be given in following days to maintain pre-operative body weight, if necessary.</li>
		<li>Monitor the animal until recovery to ensure successful surgery and assess the overall health of the animal. 
		Caution: Expect a slight hunched posture for an invasive procedure such as this, but the animal will behave normally as early as 30 minutes post-operation.</li>
		<li>Place the animal in its own cage and do not place it with other animals until fully recovered.</li>
		<li>Re-administer analgesic daily for up to 48 h&#160;postop.</li>
		<li>Place food on the floor of the cage for the animal while in recovery. Other special care is generally not needed.</li>
		<li>Examine the wound site for redness, swelling, or discharge and any other signs of infection.</li>
		<li>If necessary, remove sutures after 7 - 10 days.</li>
		<li>Perform euthanasia immediately if surgery is unsuccessful (e.g., significant vessel damage and/or blood loss) or animal does not improve with post-operative care.&#160;Euthanasia is generally performed at 48 h&#160;postop. For longer experiments, use 7-0 Prolene to ligate side branches and the IVC and 5-0 Vicryl sutures to close the abdominal cavity.
		<ol>
			<li>Perform euthanasia by anesthetization of the animal in an induction as previously described, except at 5% isoflurane. This is followed by asphyxiation with 100% CO2 while anesthetized.</li>
			<li>After confirmation of euthanasia by asphyxiation (loss of heartbeat and no breathing), perform cervical dislocation to ensure complete euthanasia.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>
2. High Frequency Ultrasound Protocol
NOTE: This protocol is adapted from the Lady Davis Institute Rodent Phenotyping Core SOP for high frequency ultrasound imaging. This protocol is carried out 24 h&#160;post-operative, but can be done sooner as long as the animal is responding well to the surgery. The protocol can be carried out at any time on healthy mice and is often done so to compare before and after surgery.
<ol>
	<li>Preparation of Animal
	<ol>
		<li>Place the animal in an induction chamber and anesthetize the animal with a mixture of 100% oxygen and 2.5% isoflurane.</li>
		<li>Turn on heart rate and temperature monitor. Position heat lamp above table to keep animal warm during procedure.</li>
		<li>Remove the animal from the induction chamber and apply eye lubricant to prevent corneal desiccation. Position the animal on the analysis platform and affix the anesthetic tube to the animal&#39;s mouth/nose.</li>
		<li>Warm ultrasound gel to 37 &#176;C and place the gel on the abdomen of the animal. The ultrasound gel is warmed using a water-heating system that pumps warm water through coils that are wrapped around the bottle of gel.</li>
		<li>For heart rate measurements (if needed), put electrode gel on the 4 electrodes of the analysis platform and fix the paws of the animal to the platform/electrodes with surgical tape. Place the animal in a supine position for imaging of the IVC. The ultrasound imaging system includes an analysis platform that is warmed and contains electrodes for monitoring the animal.</li>
		<li>For temperature measurements, put electrode gel on thermometer and insert into the rectum of the animal.</li>
		<li>Adjust heat lamp and isoflurane as necessary to ensure that animal is comfortable, has a respiration between 30 - 70 bpm, and is kept at 37 &#176;C.</li>
		<li>If necessary, shave abdomen of animal or remove hair with hair removal cream. Apply for 1 - 2 minutes and wipe clean with gauze and distilled water. 
		NOTE: Too much hair can affect the quality of the images.</li>
		<li>Place gauze under or to the side of the animal to catch any excess ultrasound gel.</li>
	</ol>
	</li>
	<li>Imaging the IVC and Thrombus
	<ol>
		<li>Turn on the imaging software and begin a new study.</li>
		<li>Select an appropriate scanhead for imaging. The scanhead used here is for abdominal organs (Frequency: 35 MHz, Focal length: 12.8 mm).</li>
		<li>Record any relevant information about the animal. This may include animal ID, sex, weight, birthdate, strain, etc.</li>
		<li>Lower the scanhead down until it is touching the ultrasound gel on the animal.</li>
		<li>Start the scanhead probe to begin 2-D imaging of the animal. In this mode, images are presented as two dimensional, in varying shades of gray.</li>
		<li>Locate the IVC and abdominal aorta by moving the mouse platform throughout the x- and/or y-plane, while also moving the scanhead through the z-plane. 
		NOTE: Blood vessels will appear with their walls white and the blood inside nearly black. The abdominal aorta will pulsate intensely and have thicker walls, while the IVC will have thinner walls and compress/expand easily on the image screen when the scanhead is moved up and down. The ligation site will be apparent, as the vein will be dilated and the vein wall will come to a point. Any thrombus formed inside will be solid under ultrasound imaging and will not compress easily like the vein wall does. The vein will compress only above the ligation, where no thrombus is formed.</li>
		<li>Upon locating the IVC and the ligation site, center them on the focal point of the ultrasound, in order to achieve the most accurate image.
		<ol>
			<li>If the image is too dark to see clearly, adjust the gel and remove any air bubbles with a cotton tip applicator.</li>
			<li>If necessary, tilt the scanhead 45&#176; to the left or right and readjust the animal platform to see the vein clearly. This is done if there is interference with the scanhead. In this scenario, the vein needs to be imaged at a side-angle to avoid the suture. 
			NOTE: Image interference is most often caused by dark patches of skin on the animal, poor wound suture placement, or bubbles in the ultrasound gel.</li>
		</ol>
		</li>
		<li>Using the software, take photos of any desired structures and make measurements using any measurement tools. Common measurements include cross-sectional area of the thrombus or width of the ligation site.</li>
		<li>Change the imaging mode to Pulse-wave Doppler to make blood flow measurements. This mode allows the imaging of vascular blood flow and the measurement of flow velocity, among other variables.</li>
		<li>Tilt the analysis platform to lower the posterior end of the animal.</li>
		<li>Change the angle/position of the scanhead so that it will contact the gel at an angle of about 30&#176; from the posterior end of the animal.</li>
		<li>Reposition the animal platform and scanhead. Relocate the IVC and ligation if necessary.</li>
		<li>Make blood flow measurements around the ligation site to confirm stasis and take any images desired. Common measurements of blood flow include average velocity, peak velocity, or median velocity.</li>
		<li>Save any measurements or images taken.</li>
	</ol>
	</li>
	<li>Clean-up and animal recovery
	<ol>
		<li>Raise the scanhead to initial position and reposition animal platform to its initial position, as well.</li>
		<li>Remove excess gel off the animal with gauze. Do this gently so that the wound suture will not be ruptured.</li>
		<li>Remove the thermometer from the animal&#39;s rectum and take the animal off the platform.</li>
		<li>Place the animal in its cage and a 34 &#176;C incubator.</li>
		<li>Monitor the animal until recovery. The procedure is very minor and non-invasive. The animal will wake up quickly. Continue monitoring animal in the following days in accordance with section 1.6 of this protocol.</li>
		<li>Clean the animal platform with gauze and disinfectant.</li>
		<li>Wipe the scanhead clean with gauze and distilled water. 
		Caution: Clean the scanhead very gently and only with distilled water.</li>
		<li>Repeat step 2 for the next animal.
		<ol>
			<li>If done with all subjects, verify that all images and recordings have been saved. Then turn off all equipment and software.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>

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D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prostaglandin+E+synthase+is+upregulated+by+Gas6+during+cancer-induced+venous+thrombosis.">Prostaglandin E synthase is upregulated by Gas6 during cancer-induced venous thrombosis.</a> Blood. 127 (6), 769-777 (2016).</li><li>Laurance, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gas6+(Growth+Arrest-Specific+6)+Promotes+Inflammatory+(CCR2hiCX3CR1lo)+Monocyte+Recruitment+in+Venous+Thrombosis.">Gas6 (Growth Arrest-Specific 6) Promotes Inflammatory (CCR2hiCX3CR1lo) Monocyte Recruitment in Venous Thrombosis.</a> Arterioscler Thromb Vasc Biol. , (2017).</li><li>Diaz, J. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Critical+review+of+mouse+models+of+venous+thrombosis.">Critical review of mouse models of venous thrombosis.</a> Arterioscler Thromb Vasc Biol. 32 (3), 556-562 (2012).</li><li>Aghourian, M. N., Lemarie, C. A., Blostein, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+monitoring+of+venous+thrombosis+in+mice.">In vivo monitoring of venous thrombosis in mice.</a> J Thromb Haemost. 10 (3), 447-452 (2012).</li><li>Brandt, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deep+vein+thrombus+formation+induced+by+flow+reduction+in+mice+is+determined+by+venous+side+branches.">Deep vein thrombus formation induced by flow reduction in mice is determined by venous side branches.</a> Clin Hemorheol Microcirc. 56 (2), 145-152 (2014).</li><li>Diaz, J. A., Farris, D. M., Wrobleski, S. K., Myers, D. D., Wakefield, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inferior+vena+cava+branch+variations+in+C57BL/6+mice+have+an+impact+on+thrombus+size+in+an+IVC+ligation+(stasis)+model.">Inferior vena cava branch variations in C57BL/6 mice have an impact on thrombus size in an IVC ligation (stasis) model.</a> J Thromb Haemost. 13 (4), 660-664 (2015).</li><li>Luther, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Innate+Effector-Memory+T+Cell+Activation+Regulates+Post-Thrombotic+Vein+Wall+Inflammation+and+Thrombus+Resolution.">Innate Effector-Memory T Cell Activation Regulates Post-Thrombotic Vein Wall Inflammation and Thrombus Resolution.</a> Circ Res. , (2016).</li><li>Subramaniam, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinct+contributions+of+complement+factors+to+platelet+activation+and+fibrin+formation+in+venous+thrombus+development.">Distinct contributions of complement factors to platelet activation and fibrin formation in venous thrombus development.</a> Blood. 129 (16), 2291-2302 (2017).</li><li>Diaz, J. 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The authors have nothing to disclose.

There are several critical steps for successful venous thrombus formation using the stasis model. Induction of vein thrombosis is more challenging in old mice due to the accumulation of fat surrounding the inferior vena cava and the aorta. Ideally, mice undergoing this procedure should be 8 - 10-weeks-old. Great care should be taken not to induce endothelial damage in the IVC during the blunt dissection and ligature. In addition, it is crucial to keep the animal in a 34 &#176;C incubator for at least 30 minutes after the...

Venous thromboembolism (VTE), which is comprised of deep venous thrombosis (DVT) and pulmonary embolism, is the third leading cause of cardiovascular death after myocardial infarction and stroke. It is a common condition affecting 1 - 2% of the population, with an annual incidence of 1 in 5001. VTE can lead to: 1) death through pulmonary embolism; 2) post-thrombotic syndrome, characterized by chronic leg pain, swelling, and ulceration; or 3) chronic pulmonary hypertension resulting in significant chronic respiratory compromise. VTE is a multi-factorial disease and may result from stasis of blood flow, damage to vessel walls, and/or hypercoagulable states due to a disruption of the balance between the coagulation and the fibrinolytic systems, as it has been described over a hundred years ago by Virchow and is known as the Virchow&#39;s Triad.
Because in most cases it is impossible to obtain human DVT samples, researchers have developed experimental animal models of DVT. Several animals including rat2, mouse3, rabbit4, pig5, dog6, and non-human primate7 have been used. Mice can be genetically modified and are the most frequently used animal to study DVT. However, as in all animals, spontaneous DVT is not observed in mice. Thus, physical or chemical alterations of the vein wall are used to create thrombosis in mice. We have previously used the ferric chloride model to induce thrombosis in the inferior vena cava (IVC) of mice8,9,10. This model has the advantage of reliably producing occlusive thrombi within minutes and can be used to investigate the role of anti-coagulant and anti-platelet drugs during acute DVT. However, it is a terminal procedure. Thus, to study acute and chronic DVT, the stasis model is more suitable. In this model, thrombus formation is induced by the complete interruption of blood flow in the IVC, one of the factors in Virchow&#39;s triad for DVT development. This model can be used to study DVT formation and resolution, which is an advantage compared to the FeCl3 model11.
We have developed a non-invasive method to follow thrombus formation and resolution over time using a micro-imaging high-frequency ultrasound system12. We have previously demonstrated that measurement of venous thrombosis by ultrasound correlates favorably with thrombi obtained pathologically. We have confirmed in two subsequent studies that measurements obtained with ultrasound correlate with thrombus weight and thrombus area quantified by histochemistry9,10. More importantly, we have showed that high frequency ultrasound can be used to monitor the formation of deep venous thrombosis in mice12. It may also be used to quantify thrombus resolution in a non-invasive way.
Here, we will describe the protocol allowing thrombus formation using the stasis-induced thrombosis mouse model and how thrombus formation can be monitored non-invasively over time using high frequency ultrasound.

<table>
	<tbody>
		<tr>
			<td>6-0 perma-hand silk suture</td>
			<td>Ethicon</td>
			<td>706G</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical Scissors</td>
			<td>Fine Science Tools</td>
			<td>20830-00</td>
			<td></td>
		</tr>
		<tr>
			<td>Suture tying forceps</td>
			<td>Fine Science Tools</td>
			<td>20830-00</td>
			<td></td>
		</tr>
		<tr>
			<td>blunt forceps (straight and curved)</td>
			<td>Fine Science Tools</td>
			<td>20830-00</td>
			<td></td>
		</tr>
		<tr>
			<td>Needle Driver</td>
			<td>Fine Science Tools</td>
			<td>13002-10</td>
			<td></td>
		</tr>
		<tr>
			<td>Moria Spring Scissors</td>
			<td>Fine Science Tools</td>
			<td>15396-00</td>
			<td></td>
		</tr>
		<tr>
			<td>1ml syringes</td>
			<td>BD Biosciences</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>26G needles</td>
			<td>Becton Dickinson &#38; Co.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>VEVO 770 High Resolution Imaging System</td>
			<td>Visualsonics</td>
			<td>No longer sold</td>
			<td></td>
		</tr>
		<tr>
			<td>SR Buprenorphine</td>
			<td>ZooPharm</td>
			<td></td>
			<td>Given to LDI by Vet</td>
		</tr>
		<tr>
			<td>Surgery Microscope</td>
			<td>Leica</td>
			<td>Leica M651</td>
			<td></td>
		</tr>
		<tr>
			<td>Systan eye oinment</td>
			<td>Alcon</td>
			<td>288/28062-0</td>
			<td></td>
		</tr>
		<tr>
			<td>2x2 sterile Gauze</td>
			<td>CDMV</td>
			<td>#104148</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton Tip Applicators</td>
			<td></td>
			<td></td>
			<td>from JGH</td>
		</tr>
		<tr>
			<td>Transpore hypoallergenic surgical tape</td>
			<td>CDMV</td>
			<td>#7411</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasound gel (Aquasonic-100)</td>
			<td>Dufort &#38; Lavigne</td>
			<td>#AKEN4061</td>
			<td></td>
		</tr>
		<tr>
			<td>Incubator</td>
			<td></td>
			<td></td>
			<td>From JGH</td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Dispomed</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Anesthetic chamber,hoses, and adminstration equipment</td>
			<td>Dispomed</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Hair remover</td>
			<td>Nair</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Water heated hard pad</td>
			<td>Braintree Scientific, Inc.</td>
			<td>#HHP-2</td>
			<td></td>
		</tr>
		<tr>
			<td>Gaymar heater water pump TP500</td>
			<td>MATVET Inc.</td>
			<td>#R-500305</td>
			<td></td>
		</tr>
		<tr>
			<td>Infra-red heating lamp</td>
			<td>electrimat inc.</td>
			<td>#1R175R-PAR</td>
			<td></td>
		</tr>
		<tr>
			<td>Mouse rectal temperature prope</td>
			<td>emkaTECHNOLOGIES</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile water</td>
			<td></td>
			<td></td>
			<td>From JGH</td>
		</tr>
	</tbody>
</table>

deep vein thrombosis induced by stasis in mice monitored by high frequency ultrasonography

Venous thrombosis is a common condition affecting 1 - 2% of the population, with an annual incidence of 1 in 500. Venous thrombosis can lead to death through pulmonary embolism or results in the post-thrombotic syndrome, characterized by chronic leg pain, swelling, and ulceration, or in chronic pulmonary hypertension resulting in significant chronic respiratory compromise. This is the most common cardiovascular disease after myocardial infarction and ischemic stroke and is a clinical challenge for all medical disciplines, as it can complicate the course of other disorders such as cancer, systemic disease, surgery, and major trauma.
Experimental models are necessary to study these mechanisms. The stasis model induces consistent thrombus size and a quantifiable amount of thrombus. However, it is necessary to systematically ligate side branches of the inferior vena cava to avoid variability in thrombus sizes and any erroneous data interpretation. We have developed a non-invasive technique to measure thrombus size using ultrasonography. Using this technique, we can assess thrombus development and resolution over time in the same animal. This approach limits the number of mice required for quantification of venous thrombosis consistent with the principle of replacement, reduction, and refinement of animals in research. We have demonstrated that thrombus weight and histological analysis of thrombus size correlate with measurement obtained with ultrasonography. Therefore, the current study describes how to induce deep vein thrombosis in mice using the inferior vena cava stasis model and how to monitor it using high frequency ultrasound.

Stasis venous thrombosis model
In the stasis model, mice are anesthetized, and an incision is made to expose the inferior vena cava (IVC). The incision is made on the left or right side of the mouse instead of a midline laparotomy in a way that would not interfere with the ultrasound probe. The abdominal muscles and the skin are fold back to expose the IVC (Figure 1). First, side br...

Watch this Scientific Journal Video about Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography at JoVE.com

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

The present protocol describes the steps to obtain venous thrombosis using a stasis model. In addition, we are using a non-invasive method to measure thrombus formation and resolution over time.

Venous thrombosis is a common condition affecting 1 - 2% of the population, with an annual incidence of 1 in 500. Venous thrombosis can lead to death ...

deep-vein-thrombosis-induced-stasis-mice-monitored-high-frequency

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Immunology and Infection

9.8K Views.  McGill University. Dit protocol beschrijft de stappen voor het verkrijgen van veneuze trombose met behulp van een stase-model. Daarnaast zijn we met behulp van een niet-invasieve methode voor het meten van de vorming van trombose en resolutie na verloop van tijd.

Video: Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)

Currently KS is the most predominant HIV/AIDS related malignancy in Southern Africa and hence the world.1,2 It is characterized as an angioproliferative tumor of vascular endothelial cells and produces rare B cell lymphoproliferative diseases in the form of pleural effusion lymphomas (PEL) and some forms of multicentric Castleman's disease.3-5 Only 1-5% of cells in KS lesions actively support lytic replication of Kaposi's sarcoma-associated herpesvirus (KSHV), the etiological agent associated with KS, and it is clear that cellular factors must interact with viral factors in the process of oncogenesis and tumor progression.6,7 Identifying novel host-factor determinants which contribute to KS pathology is essential for developing prognostic markers for tumor progression and metastasis as well as for developing novel therapeutics for the treatment of KS.8 The accompanying video details the methods we use to identify host cell gene expression programs altered in dermal microvascular endothelial cells (DMVEC) after KSHV infection and in KS tumor tissue.9 Once dysregulated genes are identified by microarray analysis, changes in protein expression are confirmed by immunoblot and dual labeled immunofluorescence. Changes in transcriptional expression of dysregulated genes are confirmed in vitro by quantitative real-time polymerase chain reaction (qRT-PCR). Validation of in vitro findings using archival KS tumor tissue is also performed by dual labeled immunochemistry and tissue microarrays.8,10 Our approach to identifying dysregulated genes in the KS tumor tissue microenvironment will allow the development of in vitro and subsequently in vivo model systems for discovery and evaluation of potential novel therapeutic for the treatment of KS.

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV

Host cell factors play a critical role in the establishment and maintenance of Kaposi's sarcoma (KS). We outline methods to identify host cell factors altered in KSHV-infected DMVEC cells, and in KS tumor tissue. Cellular genes altered by virus will serve as potential target(s) for novel therapeutics.

Currently KS is the most predominant HIV/AIDS related malignancy in Southern Africa and hence the world.1,2 It is characterized as an angioproliferative ...

Granulocyte-dependent Autoantibody-induced Skin Blistering

Autoimmune phenomena occur in healthy individuals, but when self-tolerance fails, the autoimmune response may result in specific pathology. According to Witebsky's postulates, one of the criteria in diagnosing a disease as autoimmune is the reproduction of the disease in experimental animals by the passive transfer of autoantibodies. For epidermolysis bullosa acquisita (EBA), a prototypic organ-specific autoimmune disease of skin and mucous membranes, several experimental models were recently established. In the animal model described in our present work, purified IgG antibodies against a stretch of 200 amino acids (aa 757-967) of collagen VII are injected repeatedly into mice reproducing the blistering phenotype as well as the histo- and immunopathological features characteristic to human EBA 1. Full-blown widespread disease is usually seen 5-6 days after the first injection and the extent of the disease correlates with the dose of the administered collagen VII-specific IgG. The tissue damage (blister formation) in the experimental EBA is depending on the recruitment and activation of granulocytes by tissue-bound autoantibodies 2,-4. Therefore, this model allows for the dissection of the granulocyte-dependent inflammatory pathway involved in the autoimmune tissue damage, as the model reproduces only the T cell-independent phase of the efferent autoimmune response. Furthermore, its value is underlined by a number of studies demonstrating the blister-inducing potential of autoantibodies in vivo and investigating the mechanism of the blister formation in EBA 1,3,-6. Finally, this model will greatly facilitate the development of new anti-inflammatory therapies in autoantibody-induced diseases. Overall, the passive transfer animal model of EBA is an accessible and instructive disease model and will help researchers to analyze not only EBA pathogenesis but to answer fundamental biologically and clinically essential autoimmunity questions.

In the animal model described in our present work, purified IgG antibodies against a stretch of 200 amino acids (aa 757-967) of collagen VII are injected repeatedly into mice reproducing the blistering phenotype as well as the histo- and immunopathological features characteristic to human epidermolysis bullosa acquisita (EBA)1.

Autoimmune phenomena occur in healthy individuals, but when self-tolerance fails, the autoimmune response may result in specific pathology. According to ...

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

There are many different animal models available for studying the pathogenesis of human inflammatory bowel diseases (IBD), each with its own advantages and disadvantages. We describe here an experimental colitis model that is initiated by adoptive transfer of syngeneic splenic CD4+CD45RBhigh T cells into T and B cell deficient recipient mice. The CD4+CD45RBhigh T cell population that largely consists of na&#239;ve effector cells is capable of inducing chronic intestinal inflammation, closely resembling key aspects of human IBD. This method can be manipulated to study aspects of disease onset and progression. Additionally it can be used to study the function of innate, adaptive, and regulatory immune cell populations, and the role of environmental exposures, i.e., the microbiota, in intestinal inflammation. In this article we illustrate the methodology for inducing colitis with a step-by-step protocol. This includes a video demonstration of key technical aspects required to successfully develop this murine model of experimental colitis for research purposes.

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.

There are many different animal models available for studying the pathogenesis of human inflammatory bowel diseases (IBD), each with its own advantages ...

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in vivo is crucial for understanding the molecular basis of leukocyte transendothelial migration and interaction with vascular endothelium. One powerful approach involves intravital microscopy on transgenic mice expressing fluorescent proteins in cells of interest.
Here we present a protocol for imaging monocytes and neutrophils in the CX3CR1gfp/wt mouse i.v. injected with orange dye-labeled neutrophils with an inverted confocal microscope. Time-lapse movies gathered from 30 min&#160;to several hours of imaging allow the analysis of leukocyte behavior in mesenteric veins under both steady state and inflammatory conditions. We also describe the steps to locally induce blood vessel inflammation with TLR2/TLR1 agonist Pam3SK4 and monitor the subsequent recruitment of neutrophils and monocytes.
The presented technique can also be used to monitor other populations of leukocytes and investigate molecules implicated in leukocyte recruitment or trafficking using other stimuli or transgenic mice.

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in ...

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays to be run in parallel on a single plate without the typical drawbacks of combining reactions together. We designed and evaluated a panel based on this principle to rapidly identify the presence of common disease agents in dogs and horses with acute respiratory illness. This manuscript describes a nanoscale diagnostic PCR workflow for sample preparation, amplification, and analysis of target pathogen sequences, focusing on procedures that are different from microliter scale reactions. In the respiratory panel presented, 18 assays were each set up in triplicate, accommodating up to 48 samples per plate. A universal extraction and pre-amplification workflow was optimized for high-throughput sample preparation to accommodate multiple matrices and DNA and RNA based pathogens. Representative data are presented for one RNA target (influenza A matrix) and one DNA target (equine herpesvirus 1). The ability to quickly and accurately test for a comprehensive, syndrome-based group of pathogens is a valuable tool for improving efficiency and ergonomics of diagnostic testing and for acute respiratory disease diagnosis and management.

High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.

Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays to be run in parallel on a single plate without the typical drawbacks of ...

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

While it is recognized that aquaporin-4 (AQP4)-specific T cells and antibodies participate in the pathogenesis of neuromyelitis optica (NMO), a human central nervous system (CNS) autoimmune demyelinating disease, creation of an AQP4-targeted model with both clinical and histologic manifestations of CNS autoimmunity has proven challenging. Immunization of wild-type (WT) mice with AQP4 peptides elicited T cell proliferation, although those T cells could not transfer disease to na&#239;ve recipient mice. Recently, two novel AQP4 T cell epitopes, peptide (p) 135-153 and p201-220, were identified when studying immune responses to AQP4 in AQP4-deficient (AQP4-/-) mice, suggesting T cell reactivity to these epitopes is normally controlled by thymic negative selection. AQP4-/- Th17 polarized T cells primed to either p135-153 or p201-220 induced paralysis in recipient WT mice, that was associated with predominantly leptomeningeal inflammation of the spinal cord and optic nerves. Inflammation surrounding optic nerves and involvement of the inner retinal layers (IRL) were manifested by changes in serial optical coherence tomography (OCT). Here, we illustrate the approaches used to create this new in vivo model of AQP4-targeted CNS autoimmunity (ATCA), which can now be employed to study mechanisms that permit development of pathogenic AQP4-specific T cells and how they may cooperate with B cells in NMO pathogenesis.

Here, we present a protocol to induce paralysis and opticospinal inflammation by transfer of aquaporin-4 (AQP4)-specific T cells from AQP4-/- mice into WT mice. In addition, we demonstrate how to use serial optical coherence tomography to monitor visual system dysfunction.

While it is recognized that aquaporin-4 (AQP4)-specific T cells and antibodies participate in the pathogenesis of neuromyelitis optica (NMO), a human ...

Stenosis of the Inferior Vena Cava: A Murine Model of Deep Vein Thrombosis

Deep vein thrombosis (DVT) and its devastating complication, pulmonary embolism, are a severe health problem with high mortality. Mechanisms of thrombus formation in veins remain obscure. Lack of mobility (e.g., after surgery or long-haul flights) is one of the main factors leading to DVT. The pathophysiological consequence of the lack of mobility is blood flow stagnation in venous valves. Here, a model is described that mimics such flow disturbance as a thrombosis-driving factor. In this model, partial flow restriction (stenosis) in the inferior vena cava (IVC) is created. Closure of about 90% of the IVC lumen for 48 h results in development of thrombi structurally similar to those in humans. The similarities are: i) most of the thrombus volume is red, i.e., consists of red blood cells and fibrin, ii) presence of a white part (lines of Zahn), iii) non-denuded endothelial monolayer, iv) elevated plasma D-Dimer levels, and v) possibility to prevent thrombosis by low molecular weight heparin. Limitations include variable size of thrombi and the fact that a certain percentage of wild-type mice (0 - 35%) may not produce a thrombus. In addition to visual observation and measurement, thrombi may be visualized by non-invasive technologies, such as ultrasonography, which allows for monitoring the dynamics of thrombus development. At shorter time points (1 - 6 h), intravital microscopy may be applied to directly observe events (e.g., recruitment of cells to the vessel wall) preceding thrombus formation. Use of this method by several teams around the world has made it possible to uncover basic mechanisms of DVT initiation and identify potential targets that might be beneficial for its prevention.

We describe here stenosis in the inferior vena cava as a murine model of deep vein thrombosis. This model recapitulates blood flow restriction, one of the major triggers of venous thrombosis in humans.

Deep vein thrombosis (DVT) and its devastating complication, pulmonary embolism, are a severe health problem with high mortality. Mechanisms of thrombus ...

Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization

The measurement of metabolic and endocrinal markers during physical activity is of relevance to understanding the physiological implications of different exercise modalities. During some exercise modalities (e.g., high-intensity interval exercise), blood metabolites and hormonal levels change in short periods of time. In the present study, we describe a method to catheterize the antecubital vein, which allows the collection of several blood samples during exercise. Insulin and venous lactate concentrations were measured during high-intensity exercise by the application of the described method. The exercise consisted of three 30 s bouts of high-intensity exercise separated by 4 min of recovery. After the last recovery period, a Wingate test was performed. Blood samples from the antecubital vein were obtained before and after each 30 s bout and before and after the Wingate test. As a result, it was possible to evaluate the plasma insulin and venous blood lactate variations during the exercise.

Here we present a protocol to obtain several blood samples from the antecubital vein during high-intensity interval training. This protocol may be useful for the measurement of blood metabolites and endocrinal markers during exercise.

The measurement of metabolic and endocrinal markers during physical activity is of relevance to understanding the physiological implications of different ...

Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls

Epifluorescence intravital video microscopy (IVM) of blood vessels is an established method to evaluate the activation of immune cells and their ability to role and adhere to the endothelial layer. Visualization of circulating cells by injection of fluorescent dyes or fluorophore-coupled antibodies is commonly used. Alternatively, fluorescent reporter mice can be used. Interactions of leukocytes, in particular lysozyme M+ (LysM+) monocytes, with the vessel wall play pivotal roles in promoting vascular dysfunction and arterial hypertension. We here present the technique to visualize and quantify leukocyte rolling and adhesion in carotid arteries in angiotensin II (AngII)-induced hypertension in mice by IVM.
The implantation of a catheter damages the vascular wall and leads to altered blood cell responses. We compared different injection techniques and administration routes to visualize leukocytes in a LysMCre+IRG+ mouse with widespread expression of red fluorescent protein and conditional expression of green fluorescent protein in LysM+ cells. To study LysM+ cell activation, we used AngII infused mice in which rolling and adhesion of leukocytes to the endothelium is increased. We either injected acridine orange using a jugular catheter or directly though the tail vein and compared the amount of rolling and adhering cells. We found that jugular catheter implantation per se increased the number of rolling and adhering LysM+ cells in sham-infused LysMCre+IRG+ mice compared to controls. This activation was augmented in AngII-infused mice. Interestingly, injecting acridine orange directly through the tail vein did not increase LysM+ cell adhesion or rolling in sham-infused mice. We thereby demonstrated the importance of transgenic reporter mice expressing fluorescent proteins to not interfere with in vivo processes during experimentation. Furthermore, tail vein injection of fluorescent tracers might be a possible alternative to jugular catheter injections.

This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.

Epifluorescence intravital video microscopy (IVM) of blood vessels is an established method to evaluate the activation of immune cells and their ability ...

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Rheumatoid arthritis (RA) is a complex chronic inflammatory autoimmune disease. The pathogenesis of the disease is related to invariant natural killer T (iNKT) cells. Patients with active RA present fewer iNKT cells, defective cell function, and excessive polarization of Th1. In this study, an RA animal model was established using a mixture of hGPI325-339 and hGPI469-483 peptides. The iNKT cells were obtained by in vivo induction and in vitro purification, followed by infusion into RA mice for adoptive immunotherapy. The in vivo imaging system (IVIS) tracking revealed that iNKT cells were mainly distributed in the spleen and liver. On day 12 after cell therapy, the disease progression slowed down significantly, the clinical symptoms were alleviated, the abundance of iNKT cells in the thymus increased, the proportion of iNKT1 in the thymus decreased, and the levels of TNF-&#945;, IFN-&#947;, and IL-6 in the serum decreased. Adoptive immunotherapy of iNKT cells restored the balance of immune cells and corrected the excessive inflammation of the body.

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

This protocol uses G6PI mixed peptides to construct rheumatoid arthritis models that are closer to that of human rheumatoid arthritis in CD4+ T cells and cytokines. High purity invariant natural killer T cells (mainly iNKT2) with specific phenotypes and functions were obtained by in vivo induction and in vitro purification for adoptive immunotherapy.

Rheumatoid arthritis (RA) is a complex chronic inflammatory autoimmune disease. The pathogenesis of the disease is related to invariant natural killer T ...

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

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