Creating a New CryoSPARC v3 Project and Importing Data
1:34
CryoSPARC v3: Movie Alignment and Contrast Transfer Function (CTF) Estimation
2:52
CryoSPARC v3: Manual and Template-Based Particle Picking
4:21
CryoSPARC v3: 2D Classification
4:52
CryoSPARC v3: Ab-initio Reconstruction and Homogeneous Refinement
5:31
Exporting Particle Coordinates from CryoSPARC v3 and Importing Them to RELION-3 Using PyEM
5:56
RELION-3: Particle Extraction and 2D Classification, 3D Refinement, Mask Creation, and Post-Processing
7:41
RELION-3: Polishing Training, Particle Polishing, CTF, and Per-Particle Refinements
9:15
Transferring RELION-3 Particle Coordinates and 3D Map to Scipion 3
10:05
Scipion 3: High-Resolution Refinement
11:21
Results: Using cryoSPARC v3, RELION-3, and Scipion 3 to Obtain a High-Resolution 3D Reconstruction of the Adeno-Associated Virus
12:52
Conclusion
필기록
Single-particle cryo-electron microscopy is the method for structured determinations of macromolecules at near atomic resolution. Multiple software packages are available for image processing and structure calculations, yet the result on 3D maps o
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This article describes how to effectively utilize three cryo-EM processing platforms, i.e., cryoSPARC v3, RELION-3, and Scipion 3, to create a single and robust workflow applicable to a variety of single-particle data sets for high-resolution structure determination.