Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Podsumowanie

Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.

Streszczenie

Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) is a method used for the identification of open (accessible) regions of chromatin. These regions represent regulatory DNA elements (e.g., promoters, enhancers, locus control regions, insulators) to which transcription factors bind. Mapping the accessible chromatin landscape is a powerful approach for uncovering active regulatory elements across the genome. This information serves as an unbiased approach for discovering the network of relevant transcription factors and mechanisms of chromatin structure that govern gene expression programs. ATAC-seq is a robust and sensitive alternative to DNase I hypersensitivity analysis coupled with next-generation sequencing (DNase-seq) and formaldehyde-assisted isolation of regulatory elements (FAIRE-seq) for genome-wide analysis of chromatin accessibility and to the sequencing of micrococcal nuclease-sensitive sites (MNase-seq) to determine nucleosome positioning. We present a detailed ATAC-seq protocol optimized for human primary immune cells i.e. CD4+ lymphocytes (T helper 1 (Th1) and Th2 cells). This comprehensive protocol begins with cell harvest, then describes the molecular procedure of chromatin tagmentation, sample preparation for next-generation sequencing, and also includes methods and considerations for the computational analyses used to interpret the results. Moreover, to save time and money, we introduced quality control measures to assess the ATAC-seq library prior to sequencing. Importantly, the principles presented in this protocol allow its adaptation to other human immune and non-immune primary cells and cell lines. These guidelines will also be useful for laboratories which are not proficient with next-generation sequencing methods.

Wprowadzenie

ATAC-seq^1,2 is a robust method that enables identification of regulatory³ open chromatin regions and nucleosome positioning. This information is applied for inferring the location, identity, and activity of transcription factors. The method's sensitivity for measuring quantitative variations in chromatin structure allows the study of the activity of chromatin factors, including chromatin remodelers and modifiers, as well as the transcriptional activity of RNA polymerase II¹. Thus ATAC-seq provides a powerful and unbiased approach for deciphering mechanisms that govern transcriptional regulation in any cell type of interest. We describe the adaptation of ATAC-seq to primary human Th1 and Th2 cells.

In ATAC-seq, hyperactive Tn5 transposase loaded with adaptors for next-generation sequencing (NGS) couples DNA fragmentation with tagging of DNA with adaptors (i.e., the "tagmentation" process)¹. Following PCR amplification, the resulting DNA libraries are ready for next-generation sequencing (Figure 1). The preferential tagmentation of accessible chromatin is detected by the analysis of local enrichment of ATAC-seq sequencing reads.

The short experimental procedure and requirement for less starting material, relative to other methods for measuring chromatin accessibility and nucleosomal positioning such as DNase-seq⁴, FAIRE-seq⁵, and MNase-seq⁶, has promoted the use of ATAC-seq in multiple biological systems including human primary cells^1,7 and clinical samples⁸, as well as unicellular organisms⁹, plants¹⁰, fruit flies¹¹, and various mammals¹².

The identity of transcription factors which are bound to accessible loci can be uncovered by analyzing the enrichment of their binding sequence motifs or combining ATAC-seq with chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq). This approach enabled the identification of lineage-specific transcription factors important for hematopoiesis in mouse¹³. The unbiased and global nature of ATAC-seq allows studying gene regulation in organisms for which reagents such as antibodies for ChIP analysis are not available. For example, evolutionary variations in cis-regulatory regions have been identified by studying cranial neural crest cells from humans and chimpanzees¹⁴, developmental variations in regulatory elements during early mouse embryogenesis¹⁵, changes in the regulatory landscape during a life cycle of unicellular C. owzarzaki⁹, and the evolution of promoters and enhancers across 20 mammal species¹².

ATAC-seq has also been instrumental for measuring chromatin accessibility in single cells, thus revealing variability within cell populations, which usually evades genome-wide studies^7,16. In addition, ATAC-seq can be used to study changes that occur in DNA regulatory regions in disease conditions, in which samples are rare. For example, ATAC-seq can be used to study changes in the regulatory landscape during the onset of acute myeloid leukemia (AML)¹⁷ or Ras-driven oncogenesis¹¹.

Protokół

All the procedures were approved by institutional review board of Bar Ilan University and the protocol follows guidelines provided by the committee approving the experiments.

1. Purification of Naïve Human CD4+ Cells and Polarization to T Helper 1 (Th1) and Th2 Cells

Note: Here we describe the procedure starting from frozen human peripheral blood mononuclear cells (PBMCs). The first step consists of isolating CD4+ cells using microbeads and columns that usually give us more than 95% of CD4+ cells. However, this step may vary according to the preferred protocol in each lab. The protocol for T cell activation and polarization was modified from Jenner et al. (2009)¹⁸. Isolation of CD4+ cells from 10 million PBMCs gives rise to 4 - 6 million of CD4+ cells. They are split into two flasks and grown under Th1 and Th2 polarizing conditions yielding 3-5 million Th1 and Th2 cells within just a week.

Note: Cool down the centrifuge to 4 °C before starting.

1. Thaw 1 mL of human PBMCs (10⁷ cells) in a 50 mL tube containing 10 mL of RPMI medium supplemented with 1% Penicillin-Streptomycin, 2 mM L-glutamine and 10% heat-inactivated fetal bovine serum. Centrifuge at 500 x g for 5 min. Remove the supernatant and resuspend the cells with a sterile 25 mL pipette in 15 mL of supplemented RPMI medium. Transfer the cells (with sterile 25 mL pipette) to a T75 culture flask.
2. Leave the cells overnight in a humidified incubator (37 °C, 5% CO₂).
3. Transfer the floating cells with a sterile 25 mL pipette to 50 mL tube. Determine cell number and viability by trypan blue exclusion.
4. Isolate CD4+ cells from 10 million live non-adherent PBMCs by positive selection using CD4+ microbeads and columns according to the manufacturer's recommendations (see Table of Materials/Equipment) with the following modifications: 10⁷ PBMCs are labeled with 30 µL of CD4 microbeads in 120 µL of 0.5% BSA in PBS.
5. Activate the CD4+ T cells for 72 h by rhIL-2 (10 ng/mL), plate-bound anti-CD3 (5 µg/mL) and soluble anti-CD28 (2 µg/mL). For Th1 polarization, add rhIL-12 (20 ng/mL) and anti-IL-4 (10 µg/mL). For Th2 polarization, add rhIL-4 (40 ng/mL) and anti-IFN-γ (10 µg/mL).
6. Culture the cells for additional 7 days in the presence of rhIL-2 (10 ng/mL) and the same polarizing cytokines (rhIL-12 for Th1 and rhIL-4 for Th2).

2. Nuclei Isolation

NOTE: ATAC-seq is performed with intact nuclei. Lysis buffer containing 0.05% nonylphenyl polyethylene glycol (see Table of Materials/Equipment) was calibrated for isolating nuclei from primary human Th1 and Th2 cells. We recommend calibrating this step with the laboratory reagents and cells. An excess of intact cells from insufficient detergent decreases the efficiency of the transposition reaction. Cell lysis efficiency is determined by the number of nuclei (trypan blue positive cells) relative to the total number of cells.
NOTE: Prepare the lysis buffer (10 mM Tris-HCl, pH 7.5, 10 mM NaCl, 3 mM MgCl₂). Cool down a centrifuge with a swing-bucket rotor to 4 °C. Pelleting the cells in a swing-bucket centrifuge instead of a fixed angle centrifuge reduces cell/nuclei loss. To avoid nuclei or cell loss, pipet carefully when discarding the supernatant.

1. Add fresh nonylphenyl polyethylene glycol (to a final concentration of 0.05%) and 100x protease inhibitors (to a final concentration of 1x) to the cold lysis buffer immediately before use. Keep the buffer on ice.
2. Count T cells to determine their amount and viability using trypan blue method. Viability that is lower than 90% results in higher non-specific digestion.
3. Transfer 0.5 x 10⁶ T cells (Th1 or Th2) to 1.5 mL microtubes. Spin down at 500 x g for 5 min at 4 °C.
4. Resuspend the cell pellet in 1 mL of cold phosphate-buffered saline (PBS) solution. Spin down at 500 x g for 5 min at 4 °C.
5. Resuspend the cell pellet in 1 mL of cold lysis buffer (containing nonylphenyl polyethylene glycol and protease inhibitors). Keep the tube on ice. Pipette gently to avoid disrupting the nuclei.
6. Quickly take 10 µL and count the cells with an automated cell counter while the microtube with lysed cells is on ice. This step should not exceed five minutes to avoid damaging the nuclei. At least 80% of the cells should be lysed.
7. Continue immediately with the transposition reaction. Keep the prepared nuclei on the ice.

3. Transposition reaction

NOTE: In this step, isolated nuclei are incubated with prokaryotic Tn5 transposase (TDE1) loaded with adapters for NGS sequencing. Hyperactive Tn5 simultaneously fragments DNA and ligates adapters into accessible regions of the genome (tagmentation process). The ratio between nuclei and Tn5 transposase is critical for preferential cleavage at accessible chromatin. This protocol is calibrated for 100,000 nuclei in a 100 μL reaction volume. However, the reaction can be scaled down.

1. Set temperature in a thermal shaker to 37 °C.
2. Transfer 100,000 nuclei to a 1.5 mL microtube.
3. Centrifuge at 500 x g for 10 min at 4 °C and gently remove the supernatant.
4. Add the transposition reaction components to the nuclei as specified in Table 1.
5. Resuspend by gentle pipetting.
6. Incubate the transposition reaction in a thermal shaker at 37 °C for 30 min with gentle shaking (500 rpm).
   Note: DNA Cleanup is performed by solid-phase reversible immobilization beads¹⁹ (see Table of Materials/Equipment) or PCR purification columns. At the end of the cleanup, elute the DNA fragments in 20 µL of 10 mM Tris-HCl, pH 8. Avoid EDTA in the elution buffer.

4. PCR Enrichment of ATAC-seq Libraries

NOTE: This step is aimed to amplify the ATAC-seq library i.e., DNA fragments with inserted adapters. To allow mixing of several ATAC-seq libraries in the same next-generation sequencing lane ("multiplexing") use unindexed Primer 1 (Ad1_noMx)¹ for all samples, and a different indexed (barcoded) Primer 2 (Ad 2.1 - 2.24)¹ for each sample. The primer sequences are provided in the supplemented Table of Materials/Equipment.

1. Initial PCR amplification
   NOTE: The working concentration of Primer 1 (Ad1_noMx) and Primer 2 is 25 µM. All the primers are diluted from original stock of 100 µM to 25 µM. In all of the PCR reactions, use Primer 1 (Ad1_noMx) and only one of the indexed Primer 2.
   1. Add the components of the PCR reaction as specified in Table 2 to a sterile PCR tube.
   2. Place PCR tube into a thermal cycler and perform PCR amplification using the cycling conditions detailed in Table 3.
2. Assessment of number of additional amplification cycles
   NOTE: The number of additional PCR cycles should yield sufficient amount of library fragments for a successful next-generation sequencing run, while minimized to avoid GC and size bias²⁰. The determination of the number of PCR cycles (N) required for optimal library fragment amplification is done by quantitative PCR (qPCR).
   1. Dilute Primers 1 (Ad1_noMx) and 2 (used for initial library amplification) from 25 µM to 6.25 µM.
   2. Add the components to optical PCR tubes or a plate as stated in Table 4.
   3. Place in a qPCR instrument and cycle as specified in Table 5.
   4. To estimate the required number of additional amplification cycles (N), plot cycle number on the x-axis and relative fluorescence (RFU) on the y-axis.
   5. The number of additional amplification cycles (N) is 1/3 of the number of cycles at which the qPCR reaction reached the plateau. Figure 2 provides examples for three ATAC-seq libraries that reached plateau at ~2,350 relative fluorescence units, RFU (thick green line). The number of PCR cycles in which one third of the maximal amount (783 RFU, marked on y-axis) is amplified corresponds to 8 cycles for two of the libraries (red and blue amplification curves) and 9 PCR cycles for the third library (pink).
3. Final PCR amplification
   1. Amplify the remaining 45 µL of the PCR reaction. Place a PCR tube containing amplification reaction from step 4.1.2 in a thermal cycler. Run the PCR program described in Table 6. Use the previously determined (step 4.2.5) number of amplification cycles (N).

5. Size Selection of ATAC-Seq Libraries

NOTE: In our experience, size selection of amplified ATAC-seq libraries improves next-generation sequencing results because it eliminates high molecular weight library fragments from the final ATAC-seq library.
NOTE: Allow the magnetic beads to warm to room temperature 30 min before use.
Prepare fresh 70% ethanol in nuclease-free water.

1. Resuspend the magnetic beads by mixing.
2. Add nuclease-free water to the ATAC-seq libraries (obtained in step 4.3.1.) and bring up to 100 µL.
3. Add 50 µL (0.5x) of resuspended DNA-binding magnetic beads to 100 µL of amplified libraries. Mix by pipetting up and down at least 10 times. Incubate samples for 5 min at room temperature. If necessary, quickly spin down the microtubes.
4. Place the tube on an appropriate magnetic stand for 2 min to separate the magnetic beads from the supernatant. After 2 min, transfer the supernatant to a new microtube.
5. Measure the volume of the supernatant by pipetting and add 0.7x of magnetic beads. Mix by pipetting up and down at least 10 times.
6. Incubate 5 min at room temperature. Place on a magnetic stand for 2 min.
7. After the 2 min incubation, discard the supernatant. Add 200 µL freshly made 70% ethanol to wash the beads while the tubes are on the magnetic stand.
8. Keep the microtube on the magnet for 30 s and then discard the ethanol.Repeat step 5.7.for two final ethanol washes.
9. Completely remove the remaining ethanol and let the beads air-dry for 5 min while the tube is on the magnet. If necessary, briefly spin the microtube. Remove the traces of ethanol with a p10 pipette tip.
10. Remove the microtube from the magnet and add 22 µL of 10 mM Tris-HCl, pH 8. Do not elute the ATAC-seq libraries in buffer containing EDTA.
11. Incubate the tube for 2 min at room temperature and then place on the magnetic stand.
12. When the solution is clear, transfer 20 µL of eluted libraries to a new sterile microtube.
13. Store the size selected ATAC-seq libraries at -20 °C.

6. Quality Analysis of the ATAC-Seq Libraries

1. Validation of the quality of ATAC-seq libraries by Real-Time PCR
   NOTE: It is important to assess the signal to noise ratio of the ATAC-seq libraries prior to next-generation sequencing. This is done by determining the relative amount of DNA fragments from accessible and inaccessible loci using quantitative PCR (qPCR). The inaccessible loci (negative control, chr1:48,137,860-48,137,934 and chr1:193,093,748-193,093,827) are amplified by primer pairs 1 and 2. The accessible loci (positive control, chr19:30,336,166-30,336,253 and chr19:11546154-11546237) are amplified by primer pairs 3 and 4. Positive and negative loci were defined from chromatin accessibility (DHS-seq) profiles of human CD4+ cells (ENCODE accessions ENCSR000EQE and ENCSR000EQG). Negative primer 1 is located in a large heterochromatic intergenic region (230 kb from TRADB2 and 88 kb from FOXD2). Negative control region 2 is in within the first intron of CDC73 gene. Positive primer pair 3 is within an open chromatin region downstream of the Cyclin E (CCNE1) gene while positive primer pair 4 is centered within the promoter of protein kinase C substrate 80K-H (PRKCSH). Importantly, these control loci show a similar pattern of accessibility in other human cell types from the ENCODE project³, suggesting that they can be applied to monitor ATAC-seq libraries from a broad spectrum of human cell types. Primer efficiency and specificity of all primer pairs was verified by qPCR on a serial dilution of genomic DNA (from human Th cells) and a melting curve analysis of obtained amplified products.
   1. Isolate genomic DNA using a commercially available kit (see Table of Materials/Equipment).
   2. Dilute the amplified ATAC-seq library 1:10 (1 µL library + 9 µL of nuclease-free water) and genomic DNA to ~5 ng/µL.
   3. Prepare reaction mixture (Table 7) for each positive and negative control primer pair, taking into account that the reactions are performed in triplicate.
   4. Incubate in qPCR thermal cycler according to the protocol recommended by qPCR master mix supplier.
   5. Analyze the results in qPCR instrument's software (see Table of Materials/Equipment). Choose genomic DNA as a control sample. The obtained values represent an enrichment of accessible regions (amplified by positive control primers). An example is shown in Table 8.
      NOTE: Estimation of the average library size and concentration: the size distribution of DNA fragments from ATAC-seq libraries is determined by high-sensitivity automated electrophoresis systems according to the manufacturer's instructions. It is advisable to measure the sample concentration on a fluorometer using dsDNA high-sensitivity kit and at least 2 µL of each DNA sample.
      NOTE: Next-generation sequencing - prior to multiplexing the libraries, calculate the molarity of each ATAC-seq library by using the formula: (ng/µL x 10⁶)/(660 x average library fragment length). Aim for > 30 million reads of each ATAC-seq library to assess open chromatin regions of human samples. If you wish to determine if the library is good enough for NGS sequencing, initially aim for ~10 million reads (5% of the sequencing lane on DNA sequencing instrument in rapid run mode). Keep in mind that to infer nucleosome positioning, paired-end sequencing is needed¹.

7. Analysis of the Obtained Next-Generation Sequencing Results

1. Infer the quality of the sequencing reads by inspecting the FastQC files, separately for every library.
2. Align the reads to human reference genome (hg19 assembly) using Bowtie²¹ software in the Unix/Linux environment. The command is 'bowtie -m 1 -q -S genome directory reads.fastq output_aligned.sam'. Genome directory stands for the folder where the genome indexes of Bowtie are stored. The parameter -m 1 is for not allowing alignment of reads to more than one locus in the genome, -q is for the input file that should be in fastq format, -S is for the output that is in SAM format.
3. Remove duplicate reads using SAMtools²² rmdup option in the Unix/Linux environment. The commands are 'samtools view -S output_aligned.sam -b | samtools sort -o -output_aligned > output_aligned.bam',
   samtools rmdup -s output_aligned.bam output_aligned _rmdup.bam'. The first command, view, changes the SAM format into BAM format which is then sorted. The option of rmdup is then applied on the sorted BAM file. Optionally one can adjust the reads for the transposon insertion site, as described in the original ATAC-seq paper¹.This is done using BEDtools commands²³ in the Unix/Linux environment. The commands are 'bamToBed -i output_aligned _rmdup.bam > output_aligned _rmdup.bed ', 'shiftBed -i output_aligned _rmdup.bed -p 4 -m -5 -g genome > output_aligned _rmdup_adjusted.bed'. The first command, bamTobed, changes the BAM format into BED format which can then be used for the shiftBed command. The genome file is a tab delimited file that contains the length of each chromosome in the genome. The file is usually added in the BEDtools directory.
4. Perform peak calling using model-based analysis of ChIP-seq (MACS2)²⁴ software in the Unix/Linux environment on the shifted BED file with the following parameters: --nomodel --extsize 75 --shift -30. These parameters are used to adjust the reads so that the transposon insertion site is in the middle of each sequencing read.

Wyniki

The final outcome of this protocol is an ATAC-seq library of typically 3 - 20 ng/µL. When run on a system for DNA integrity analysis (see Table of Materials/Equipment), they show ladder-like appearance² (Figure 3A). The average size of DNA fragments is typically ~450 - 530 bp.

Proper quality control of the ATAC-seq libraries prior to performing next-gene...

Dyskusje

The ATAC-seq protocol described here has been successfully employed for the analysis of accessible chromatin in primary cells (human Th1, Th2 cells, and B cells) as well as the cultured cell lines (MCF10A human breast cancer cells and U261 glioblastoma cells). Applying ATAC-seq to other cell types may require some protocol optimization, especially in the lysis step. If the concentration of non-ionic detergent is too high, there may be a higher percentage of mitochondrial DNA contamination. This can be reduced by decreasi...

Materiały

Name	Company	Catalog Number	Comments
50 mL tubes	Lumitron	LUM-CFT011500-P	Can be from other vendors.
Microtubes	Axygen Inc	MCT-175-C	Can be from other vendors.
25 mL serological pipettes	Corning Costar	4489	Can be from other vendors.
Tissue culture flask	Lumitron	LUM-TCF-012-250-P	Can be from other vendors.
Countes Automated Cell Counter	Invitrogen	C10227
NucleoSpin Tissue	MACHEREY-NAGEL	740952.5
Peripheral blood mononuclear cells (PBMC)	ATCC	PCS­800­011	Can be from other vendors.
RPMI 1640 Medium	Biological Industries	01-103-1A	Can be from other vendors.
L-Glutamine Solution (200 mM)	Biological Industries	03-020-1B	Can be from other vendors.
Penicillin-Streptomycin	Biological Industries	03-031-1B	Can be from other vendors.
Fetal Bovine Serum (FBS), Heat Inactivated, European Grade	Biological Industries	04-127-1	Can be from other vendors.
MACS CD4 microbeads, human	Miltenyi Biotec	130-045-101
MACS MS columns	Miltenyi Biotec	130-042-201
Anti-Human CD4 FITC	Biogems	06121-50
Mouse IgG1 Isotype Control FITC	Biogems	44212-50
Anti-Human CD3 (OKT3)	Tonbo biosciences	40-0037
Anti-Human CD28 SAFIRE Purified	Biogems	10311-25
Recombinant Human IL2	Peprotech	200-02
Recombinant Human IL4	Peprotech	200-04
Recombinant Human IL12 p70	Peprotech	200-12
In Vivo Ready Anti-Human IL-4 (MP4-25D2)	Tonbo	40-7048
LEAF Purified anti-human IFN-γ	BioLegend	506513
NaCl, analytical grade	Carlo Erba	479687	Can be from other vendors.
Magnesium chloride, Hexahydrate, molecular biology grade	Calbiochem	442611	Can be from other vendors.
EDTA	MP Biomedicals	800682	Can be from other vendors.
Tris, ultra pure, 99.9% pure	MP Biomedicals	819620	Can be from other vendors.
NP-40 alternative (Nonylphenyl Polyethylene Glycol)	Calbiochem	492016	Can be from other vendors.
Protease Inhibitors	Sigma	P2714	this protease inhibitor coctail is a powder. To make 100 x solution dilute in 1 mL of molecular-biology grade water.
Magnetic solid phase reverse immobilization beads: AMPure XP beads	Beckman	63881
PCR purification kit	HyLabs	EX-GP200	Can be from other vendors.
Nextera DNA Library Preparation Kit (TDE1 transposase and TD buffer)	Illumina	FC-121-1030
NEBNext High-Fidelity 2 x PCR Master Mix	New England BioLabs	M0541
NEBNext Q5 Hot Start HiFi PCR Master Mix	New England BioLabs	M0543
SYBR Green I	Invitrogen	S7585
CFX Connect Real-Time PCR Detection System	Bio-rad	185-5200	Can be from other vendors.
CFX Manager Software	Bio-rad	1845000
master mix for qPCR: iTaq Universal SYBR Green Supermix	Bio-rad	172-5124	Can be from other vendors.
Qubit fluorometer 2.0	Invitrogen	Q32866
Qubit dsDNA HS Assay Kit	Invitrogen	Q32854
Magnet for eppendorf tubes	Invitrogen	12321D	Can be from other vendors.
Swing bucket cooling centrifuge with the buckets for 15 mL falcon tubes and eppendorf tubes	Thermo Scientific	75004527	Could be from other vendors. It is important that it has buckets for eppendorf tubes.
Thermo-shaker	MRC		Can be from other vendors.
High Sensitivity D1000 ScreenTape	Agilent Technologies	5067-5584
High Sensitivity D1000 Reagents	Agilent Technologies	5067-5585
4200 TapeStation system	Agilent Technologies	G2991AA	Tape-based platform for  electrophoresis
High Sensitivity DNA kit	Agilent Technologies	5067-4626	Reagent for high-sensitivity TapeStation analysis
Primer name and sequence	Company
Ad1_noMX: 5'-AATGATACGGCGACCACCGAGA TCTACACTCGTCGGCAGCGTC AGATGTG-3'	IDT		Ad1-noMx: 5'-P5 sequence-transposase sequence-3'
Ad2.1_TAAGGCGA: 5'-CAAGCAGAAGACGGCATACGAG AT[TCGCCTTA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.1_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.2_CGTACTAG: 5'-CAAGCAGAAGACGGCATACGAG AT[CTAGTACG]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.2_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.3_AGGCAGAA: 5'-CAAGCAGAAGACGGCATACGA GAT[TTCTGCCT]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.3_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.4_TCCTGAGC: 5'-CAAGCAGAAGACGGCATACGAG AT[GCTCAGGA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.4_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.5_GGACTCCT: 5'-CAAGCAGAAGACGGCATACGA GAT[AGGAGTCC]GTCTCGTGGG CTCGGAGATGT-3'	IDT		Ad2.5_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.6_TAGGCATG: 5'-CAAGCAGAAGACGGCATACGA GAT[CATGCCTA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.6_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.7_CTCTCTAC: 5'-CAAGCAGAAGACGGCATACGA GAT[GTAGAGAG]GTCTCGTGGG CTCGGAGATGT-3'	IDT		Ad2.7_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.8_CAGAGAGG: 5'-CAAGCAGAAGACGGCATACGA GAT[CCTCTCTG]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.8_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.9_GCTACGCT: 5'-CAAGCAGAAGACGGCATACGA GAT[AGCGTAGC]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.9_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.10_CGAGGCTG: 5'-CAAGCAGAAGACGGCATACG AGAT[CAGCCTCG]GTCTCGTGG GCTCGGAGATGT-3'	IDT		Ad2.10_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.11_AAGAGGCA: 5'-CAAGCAGAAGACGGCATACG AGAT[TGCCTCTT]GTCTCGTGGG CTCGGAGATGT-3'	IDT		Ad2.11_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.12_GTAGAGGA: 5'-CAAGCAGAAGACGGCATACG AGAT[TCCTCTAC]GTCTCGTGGG CTCGGAGATGT-3'	IDT		Ad2.12_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.13_GTCGTGAT: 5'-CAAGCAGAAGACGGCATACGA GAT[ATCACGAC]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.13_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.14_ACCACTGT: 5'- CAAGCAGAAGACGGCATACGA GAT[ACAGTGGT]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.14_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.15_TGGATCTG: 5'- CAAGCAGAAGACGGCATACGA GAT[CAGATCCA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.15_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.16_CCGTTTGT: 5'- CAAGCAGAAGACGGCATACGA GAT[ACAAACGG]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.16_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.17_TGCTGGGT: 5'- CAAGCAGAAGACGGCATACGA GAT[ACCCAGCA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.17_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.18_GAGGGGTT: 5'-CAAGCAGAAGACGGCATACGA GAT[AACCCCTC]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.18_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.19_AGGTTGGG: 5'-CAAGCAGAAGACGGCATACGA GAT[CCCAACCT]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.19_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.20_GTGTGGTG: 5'-CAAGCAGAAGACGGCATACGA GAT[CACCACAC]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.20_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.21_TGGGTTTC: 5'-CAAGCAGAAGACGGCATACGA GAT[GAAACCCA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.21_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.22_TGGTCACA: 5'- CAAGCAGAAGACGGCATACGA GAT[TGTGACCA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.22_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.23_TTGACCCT: 5'-CAAGCAGAAGACGGCATACGA GAT[AGGGTCAA]GTCTCGTGGGC TCGGAGATGT-3'	IDT		Ad2.23_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
Ad2.24_CCACTCCT: 5'-CAAGCAGAAGACGGCATACGA GAT[AGGAGTGG]GTCTCGTGGG CTCGGAGATGT-3'	IDT		Ad2.24_expected index sequence read: 5'-P7 sequence-[index sequence]-transposase sequence-3'
F1: 5'-CCTTTTTATTTGCCCATACACTC-3'	IDT
R1: 5'-CCCAGATAGAAAGTTGGAGAGG-3'	IDT
F2: 5'-TTGAGGGATGCCATAACAGTC-3'	IDT
R2: 5'-CTGCTGAACAACATCCTTCAC-3'	IDT
F3: 5'-GGTTTGCAGGTTGCGTTG-3'	IDT
R3: 5'-AGAGGAATCTGGGAGTGACG-3'	IDT
F4: 5'-TGCTCATTCCGTTTCCCTAC-3'	IDT
R4: 5'-AGCCGGAAAGAAAGTTCCTG-3'	IDT