JoVE Logo

Zaloguj się

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

41.7K Views

11:22 min

October 29th, 2013

DOI :

10.3791/50320-v

October 29th, 2013


Przeglądaj więcej filmów

Keywords GST His Purification

Rozdziały w tym wideo

0:05

Title

1:49

Recombinant Protein Expression and Preparation of Soluble Cell Lysate

3:17

GST Affinity Purification

5:14

TALON Metal Affinity Purification

7:00

Results: Analysis of GST-His Purification

10:21

Conclusion

Powiązane Filmy

article

11:09

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

17.2K Views

article

14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

17.8K Views

article

08:12

Expression and Purification of Mammalian Bestrophin Ion Channels

8.3K Views

article

08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

1.6K Views

article

07:10

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

3.0K Views

article

11:25

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

6.5K Views

article

07:17

Purification of Hsp104, a Protein Disaggregase

17.3K Views

article

10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

17.0K Views

article

07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

14.2K Views

article

09:51

Purification of a High Molecular Mass Protein in Streptococcus mutans

5.8K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone