Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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36.8K Views
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10:38 min
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October 8th, 2013
DOI :
October 8th, 2013
•Loretta Müller1, Luisa E. Brighton1, Johnny L. Carson1,2, William A. Fischer II1,3, Ilona Jaspers1,2,4
1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill
Rozdziały w tym wideo
0:05
Title
2:05
Obtaining Biopsy of Human NECs
3:37
Seeding NECs on Plastic
4:41
Expanding the Cells in Flasks
6:00
Seeding Cells on Tissue Culture Inserts
7:53
Establishing Air Liquid Interface (ALI)
8:35
ALI Culture of NECs Produces a Differentiated Epithelium
9:43
Conclusion
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