Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
Please note that all translations are automatically generated. Click here for the English version.
2.8K Views
•
07:36 min
•
February 28th, 2021
DOI :
February 28th, 2021
•Zhe Zhang Ryan Lew*1, Jing Liu*1, Hsiao Hui Ong1, Vivian Jiayi Tan2, Annika Luukkainen3, Yew Kwang Ong1,4, Mark Thong1,4, Kia Joo Puan5, Vincent Tak Kwong Chow2,6, Kai Sen Tan1,2,6, De Yun Wang1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
Rozdziały w tym wideo
0:04
Introduction
0:50
hNEC Viral Infection and hNEC + PBMCs Co-Culture
3:30
Harvesting of PBMCs 48/72 h Post-Viral Infection
5:31
Results: Activation of T Cells Following Influenza Infection
6:56
Conclusion
Powiązane Filmy
14:48
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
26.2K Views
12:09
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
22.4K Views
09:01
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
7.9K Views
08:11
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
8.7K Views
06:27
iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease
810 Views
08:01
Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
8.3K Views
10:26
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
10.7K Views
06:32
Assessing Respiratory Immune Responses to Haemophilus Influenzae
2.0K Views
09:02
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
1.1K Views
06:03
Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
1.1K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone