Osteoclast Derivation from Mouse Bone Marrow

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24.0K Views

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06:17 min

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November 6th, 2014

DOI :

10.3791/52056-v

November 6th, 2014

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Ruth Tevlin*¹, Adrian McArdle*¹^,², Charles K.F. Chan², John Pluvinage², Graham G. Walmsley¹^,², Taylor Wearda¹^,², Owen Marecic¹^,², Michael S. Hu¹, Kevin J. Paik¹, Kshemendra Senarath-Yapa¹, David A. Atashroo¹, Elizabeth R. Zielins¹, Derrick C. Wan¹, Irving L. Weissman¹^,², Michael T. Longaker¹^,²

¹Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine, Stanford University

Podsumowanie

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Keywords Osteoclast

Monocyte macrophage Lineage

Bone Resorption

Bone Remodeling

Skeletal Remodeling

In Vitro Osteoclast Isolation

Bone Remodeling Cycle

Therapeutic Strategies

Rozdziały w tym wideo

0:05

Title

1:28

Media Preparation and Bone Marrow Isolation

3:05

Gradient Separation of Bone Marrow Cells and Cell Culturing

4:57

Results: Culturing Osteoclasts from Mouse Bone Marrow

5:56

Conclusion

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