Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry
Please note that all translations are automatically generated. Click here for the English version.
15.5K Views
•
09:28 min
•
June 4th, 2015
DOI :
June 4th, 2015
•Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Title
1:25
Collection and Digestion of Surgical Tissue Sample
2:40
Dissociation of Tissue Sample into Single Cell Suspension and Culturing Isolated Cells on Substrates with Different Rigidity
4:45
Traction Force Microscopy
5:43
Immunofluorescence Microscopy Assays
7:10
Results: Traction Cytometry and Immunofluorescence Microscopy
8:51
Conclusion
Powiązane Filmy
06:19
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
9.9K Views
10:54
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
15.2K Views
05:17
Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
8.5K Views
07:33
In vitro Organoid Culture of Primary Mouse Colon Tumors
34.9K Views
10:38
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
2.0K Views
10:33
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
11.0K Views
Isolation of Immune Cells from Primary Tumors
37.4K Views
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
19.6K Views
03:25
Test organizacji cytoszkieletu i adhezji ogniskowej: metoda oparta na immunofluorescencji do badania adhezji komórek i rozprzestrzeniania się na podłożach
1.5K Views
02:44
Test cytometrii trakcyjnej: metoda ilościowego określania siły trakcyjnej wywieranej przez komórki rakowe na ECM
1.2K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone