Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry
15.5K Views
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09:28 min
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June 4th, 2015
DOI :
June 4th, 2015
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Chapters in this video
0:05
Title
1:25
Collection and Digestion of Surgical Tissue Sample
2:40
Dissociation of Tissue Sample into Single Cell Suspension and Culturing Isolated Cells on Substrates with Different Rigidity
4:45
Traction Force Microscopy
5:43
Immunofluorescence Microscopy Assays
7:10
Results: Traction Cytometry and Immunofluorescence Microscopy
8:51
Conclusion
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