Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

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9.7K Views

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06:39 min

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October 3rd, 2018

DOI :

10.3791/58140-v

October 3rd, 2018

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Yang Jo Chung¹, Ghanwa Khawaja¹, Karen M. Wolcott², Peter D. Aplan¹

¹Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²LGI Flow Cytometry Core Facility, Center for Cancer Research, National Cancer Institute, National Institutes of Health

Podsumowanie

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Keywords Hematopoietic Stem Cell Transplantation

Myelodysplastic Syndrome

Cancer Stem Cells

Functional Analysis

Disease Initiating Cells

Adoptive Transfer

Total Body Irradiation

Bone Marrow Nucleated Cells

Flow Sorting

Biotinylated Anti lineage Antibody

APC Conjugated Anti Sca1 Antibody

Rozdziały w tym wideo

0:04

Title

0:39

Recipient Mouse Preparation and Bone Marrow Nucleated Cell (BMNC) Harvest

2:25

BMNC Fluorescence-activated Cell Sorting (FACS) and Hematopoietic Stem Cell Transplantation (HSCT)

4:52

Results: Representative HSCT Analyses

6:08

Conclusion

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