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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

10.3K Views

10:10 min

January 17th, 2019

DOI :

10.3791/58631-v

January 17th, 2019


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Nuclear Translocation

Rozdziały w tym wideo

0:04

Title

0:29

Preparation of Guests with CagedGlue-R Tags

2:17

Preparation of Hep3B Cell Sample for Microscopic Observations

2:59

Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light

4:53

Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light

5:48

Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light

7:04

Cell Viability Assay

8:20

Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells

9:51

Conclusion

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