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Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

6.7K Views

09:07 min

May 1st, 2019

DOI :

10.3791/59527-v

May 1st, 2019


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Aspergillus Nidulans

Chapters in this video

0:04

Title

0:53

Inoculum Preparation

1:34

Growth and Harvesting of Mycelia

2:36

Preparation of the Protein Extract and Equilibration of the IgG Resin

4:40

Batch Purification of TAP-tagged HDAC

6:50

Results: Analysis of the Purification and Enzymatic Activity Assay

8:29

Conclusion

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