Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

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6.7K Views

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11:00 min

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July 6th, 2019

DOI :

10.3791/59661-v

July 6th, 2019

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Shaoyang Wang¹^,², Minglie Hu², Tao Ren¹, Hao He³

¹Department of Respiratory Medicine, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, ²Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, ³School of Biomedical Engineering, Shanghai Jiao Tong University

Podsumowanie

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Single cell Operation

Cellular Signaling

HeLa Cells

Fluorescence Microscopy

Optical Manipulation

Laser Scanning

Molecular Dynamics

Experimental Protocol

Rozdziały w tym wideo

0:04

Title

1:04

Setting Up the Photostimulation System

1:48

Preparing for Activation of ERK2 by Photostimulation

2:47

Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-A Mode

4:24

Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-B Mode

5:53

Activation of Mitoflashes and Other Mitochondrial Events by Photostimulation

6:55

Delivering Femtosecond Laser Stimulation into the Target Mitochondrion Using Stim-A Mode

8:32

Results: Activation of ERK Signaling or Mitochondrial Events in Target Cells

10:26

Conclusion

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