Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

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12.8K Views

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12:22 min

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August 18th, 2019

DOI :

10.3791/59971-v

August 18th, 2019

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Titis A.K. Wardhani¹^,², Yuda Purwana Roswanjaya¹^,², Simon Dupin¹^,³, Huchen Li¹^,⁴, Sidney Linders¹, Marijke Hartog¹, Rene Geurts¹, Arjan van Zeijl¹

¹Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, ²Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), ³Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, ⁴Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

Podsumowanie

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Parasponia Andersonii

Genome Editing

Nitrogen fixing

Rhizobium Symbiosis

Transgenic Mutant Lines

Plant Growth Protocol

Seed Germination

Disinfection Technique

Experimental Platform

Greenhouse Conditions

Rozdziały w tym wideo

0:04

Title

1:08

Growth of P. andersonii Trees

3:14

Stable transformation of P. andersonii

6:40

Preparation of Rooted P. andersonii Plantlets

7:31

Nodulation of P. andersonii Plantlets in Pots

9:48

Results: Stable Transformation Procedure

11:13

Conclusion

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