Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
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10.7K Views
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09:49 min
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December 3rd, 2019
DOI :
December 3rd, 2019
•Rozdziały w tym wideo
0:05
Title
0:43
Brain Region Dissection
1:43
Mechanical Tissue Dissociation
2:40
Myelin Removal
4:25
Staining for Fluorescence-activated Cell Sorting
5:18
Index FACS Sorting
5:46
Single-cell RNA-sequencing Library Preparation
7:17
Results: Isolation of Region-Specific Microglia for Deep Single-Cell RNA Sequencing
9:16
Conclusion
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