Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

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10.7K Views

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11:11 min

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January 24th, 2020

DOI :

10.3791/60420-v

January 24th, 2020

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Chi-Hao Hsiao¹^,²^,³, Ya-Hsu Chiu⁴, Shao-Chih Chiu⁴^,⁵, Der-Yang Cho⁴^,⁶^,⁷, Liang-Ming Lee¹^,², Yu-Ching Wen¹^,², Jia-Jhe Li⁴, Ping-Hsiao Shih⁴

¹Department of Urology, Wan Fang Hospital, ²Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, ³Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, ⁴Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, ⁵Graduate Institute of Biomedical Sciences, China Medical University, ⁶Graduate Institute of Immunology, China Medical University, ⁷Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

Podsumowanie

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Cytotoxic T Cells

Cytokine induced Killer Cells

PBMC

Cancer Treatment

Cell Expansion

Standard Operating Procedure

Cell Viability

Trypan Blue Exclusion Assay

Flow Cytometry

Scatter Gating System

Immunophenotyping

Experimental Protocol

Cell Suspension

Cell Analysis

Rozdziały w tym wideo

0:04

Introduction

0:50

Immunophenotyping for Assessment of CIK Cells

2:14

CD Marker Recognition

4:12

Cytotoxic Assay

8:15

Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay

10:10

Conclusion

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