Quantification of Proliferative and Dead Cells in Enteroids
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8.3K Views
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10:47 min
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January 31st, 2020
DOI :
January 31st, 2020
•Hua-Shan Li*1, Shao-Fang Xu*1, Jian-Ying Sheng*1, Zhi-Hui Jiang1, Jing Wang1, Ning Ding1, Tao Wang1, Matthew A. Odenwald2, Jerrold R. Turner2,3, Wei-Qi He1, Hong Xu1, Juan-Min Zha1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
Rozdziały w tym wideo
0:05
Introduction
1:00
Intestinal Organoid Isolation and Culture
4:18
Flow Cytometry Analysis of EdU-Positive Cells in Enteroids
8:40
Results: IL-22 Increases Enteroid Proliferation and Promotes Cell Death in Enteroids
9:46
Conclusion
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