Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
Please note that all translations are automatically generated. Click here for the English version.
6.8K Views
•
07:37 min
•
February 21st, 2020
DOI :
February 21st, 2020
•Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Introduction
0:42
First Target Protein Staining
3:15
Antibody Stripping
3:41
Second Target Protein Staining
4:49
Fluorescence Microscopy Imaging
5:30
Results: Representative Effects of Microwave-Mediated Stripping on Antibody Cross-Reactivity
6:38
Conclusion
Powiązane Filmy
10:00
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
14.4K Views
07:13
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
12.6K Views
10:39
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
13.6K Views
06:35
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
33.6K Views
09:57
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
14.2K Views
08:14
Using Primary Neurosphere Cultures to Study Primary Cilia
9.3K Views
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
10.9K Views
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
8.1K Views
08:18
Induction and Validation of Cellular Senescence in Primary Human Cells
16.6K Views
10:03
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
12.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone