Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
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09:41 min
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May 14th, 2020
DOI :
May 14th, 2020
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Rozdziały w tym wideo
0:04
Introduction
1:08
Propagation and Sterilization
1:36
Plating
2:20
Tissue Treatment (Optional)
2:51
Harvesting
3:39
Tissue Grinding and Homogenization
4:39
Total RNA Sample Collection, Crude Extract Clearing, Bead Washing
5:34
Immunoprecipitation
6:49
Lab Supply Reconditioning
7:38
Results: Representative Cell Type-Specific GFP Expression and Translational Ribosome Affinity Purification (TRAP)/Polysome RNA Quality Assessment
8:47
Conclusion
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