Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

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7.3K Views

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10:45 min

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June 14th, 2020

DOI :

10.3791/61046-v

June 14th, 2020

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Isabel Mateos-White¹, Jaime Fabra-Beser¹, David de Agustín-Durán¹, Cristina Gil-Sanz¹

¹Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física, Universidad de Valencia

Podsumowanie

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Double In Utero Electroporation

Corticogenesis

Neuron Cells

Mammalian Development

Electroporation Technique

Rozdziały w tym wideo

0:04

Introduction

1:09

First In Utero Electroporation Surgery

3:20

Second In Utero Electroporation

4:18

Tissue Harvesting and Sectioning: Strategy A

6:09

Tissue Harvesting and Sectioning: Strategy B

7:24

Confocal Fluorescence Imaging and Analysis

8:34

Results: Representative Double Electroporation-Facilitated Cell Interaction Visualization Strategies

9:55

Conclusion

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