JoVE Logo

Zaloguj się

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

3.1K Views

11:51 min

May 11th, 2020

DOI :

10.3791/61067-v

May 11th, 2020


Przeglądaj więcej filmów

Potato Virus X

Rozdziały w tym wideo

0:04

Introduction

0:42

Short Tandem Target Mimic (STTM) Fragment Amplification

1:40

STTM Purification

2:22

T4 DNA Polymerase Reaction Mix Preparation

3:13

Potato Virus X (PVX)-Based microRNA (miRNA) Silencing (VbMS) Vector Construction

4:55

T4 DNA Polymerase Reaction Setup and STTM Cloning

7:21

Potato Virus-X-Based VbMS Assay

10:07

Results: Representative VbMS of miR165/166 in Tetraploid Potato Species

11:16

Conclusion

Powiązane Filmy

article

14:04

Viral Nanoparticles for In vivo Tumor Imaging

17.2K Views

article

08:31

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

29.3K Views

article

10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

6.2K Views

article

08:17

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

16.9K Views

article

06:34

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

52.5K Views

article

14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

10.5K Views

article

09:05

MicroRNA-based Regulation of Picornavirus Tropism

7.4K Views

article

07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

20.3K Views

article

23:21

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

41.3K Views

article

05:56

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

4.8K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone