Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

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7.8K Views

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08:33 min

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August 5th, 2020

DOI :

10.3791/61517-v

August 5th, 2020

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Lim Chee Liew¹^,², Yan Wang¹^,², Marta Peirats-Llobet¹, Oliver Berkowitz¹^,²^,³, James Whelan¹^,²^,³, Mathew G. Lewsey¹^,³

¹Department of Animal, Plant and Soil Science, AgriBio Building, La Trobe University, ²Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, ³Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building, La Trobe University

Podsumowanie

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Laser Capture Microdissection

RNA Sequencing

Spatial Transcriptomes

Temporal Transcriptomes

Plant Gene Expression

Sample Preparation

Tissue Fixation

Barley Seed Processing

Automatic Tissue Processor

Ethanol Gradient Dehydration

Xylene Immersion

Paraffin Embedding

Histological Identification

Rozdziały w tym wideo

0:04

Introduction

1:11

Tissue Fixation

1:47

Tissue Processing

2:38

Paraffin Embedding

3:21

Polyethylene Naphthalate (PEN) Membrane Slide Preparation

3:49

Sectioning

4:53

Laser-Capture Microdissection (LCM)

6:14

Results: Representative LCM Sample Acquisition and RNA Quantification

7:52

Conclusion

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