Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration

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2.6K Views

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06:50 min

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April 29th, 2021

DOI :

10.3791/61626-v

April 29th, 2021

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Kazuki Shigyou*¹, Haruka Maeoka*¹, Ryuji Igarashi²^,³^,⁴, Takuma Sugi¹

¹Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University, ²Institute for Quantum Life Science, National Institute for Quantum and Radiological Science and Technology, ³National Institute for Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, ⁴JST, PRESTO

Podsumowanie

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Calcium Imaging

Caenorhabditis Elegans

Nonlocalized Vibration

Neural Mechanisms

Nematode Growth Medium

Escherichia Coli OP50

Image splitting Optics

Software For Vibration Control

Data Analysis

Fluorescence Imaging

Mouse Macro Software

Rozdziały w tym wideo

0:04

Introduction

0:32

Cultivation of Worms and Hardware Setup for Calcium Imaging

1:46

Software Setup for Calcium Imaging

3:22

Preparation of Worms and Calcium Imaging

4:37

Data Analysis

5:23

Results: Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration

6:19

Conclusion

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