Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

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6.8K Views

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09:26 min

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January 22nd, 2021

DOI :

10.3791/62187-v

January 22nd, 2021

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Niamh E. Harrington*¹, Esther Sweeney*¹, Ilyas Alav¹^,², Freya Allen¹, John Moat¹^,³, Freya Harrison¹

¹School of Life Science, University of Warwick, ²Institute of Microbiology and Infection, University of Birmingham, ³Warwick Antimicrobial Screening Facility, School of Life Science, University of Warwick

Podsumowanie

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Antibiotic Efficacy Testing

Ex Vivo Model

Pseudomonas Aeruginosa

Staphylococcus Aureus

Biofilms

Cystic Fibrosis Lung

Candidate Drugs

Antibiotic Susceptibility Testing

Tissue Processing

Bronchiole Preparation

DMEM RPMI 1640

Sterile Techniques

Biofilm Destruction

Rozdziały w tym wideo

0:04

Introduction

0:52

Dissection and Infection of Ex Vivo Pig Lung (EVPL) Tissue

4:45

Determination of Antibiotic Efficacy

6:39

Results: Screening Bacterial Isolates for Antibiotic Susceptibility with the EVPL Model

8:49

Conclusion

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