An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Please note that all translations are automatically generated. Click here for the English version.

2.5K Views

•

11:02 min

•

August 13th, 2021

DOI :

10.3791/62693-v

August 13th, 2021

•

Tram P. Le*¹, Xiaolei Zhao*¹, Shannon Erhardt¹^,², Jianhua Gu³, Huie Wang³, Tina O. Findley¹, Jun Wang¹^,²

¹Department of Pediatrics, McGovern Medical School, The University of Texas Health Science Center at Houston, ²The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, The University of Texas Health Science Center at Houston, ³SEM AFM Core in the Houston Methodist Hospital Research Institute

Podsumowanie

Przeglądaj więcej filmów

Aminopropyl Triethoxysilane APTS

Glutaraldehyde

Dichloromethane Silane DCMS

Siliconized Cover Slips

Acrylamide Gel Solution

Rozdziały w tym wideo

0:05

Introduction

0:34

Preparation of Aminosilane-Coated and Siliconized Glass Coverslips

3:13

Preparation of Hydrogels

6:40

Molecular Analysis of Stiffness via Immunofluorescence Staining

8:04

Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System

10:31

Conclusion

Powiązane Filmy

11:22

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

13.4K Views

09:57

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick^GFPNeural Tube Grafting and Carbocyanine Dye DiI Injection

8.1K Views

07:13

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

12.5K Views

08:05

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

10.2K Views

08:46

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

11.6K Views

10:13

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

7.5K Views

09:49

Single-cell Photoconversion in Living Intact Zebrafish

5.9K Views

06:21

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

9.2K Views

09:29

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

10.7K Views

12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

7.1K Views