Preparation of Aminosilane-Coated and Siliconized Glass Coverslips
3:13
Preparation of Hydrogels
6:40
Molecular Analysis of Stiffness via Immunofluorescence Staining
8:04
Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System
10:31
Conclusion
Transcription
The method aids in the study of the mechanical signaling of the neural crest cells and its interaction the with chemical signals. It can also aid in the molecular and genetic mechanisms of the neural crest development in diseases. Transfer the gla
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Detailed step-by-step protocols are described here for studying mechanical signals in vitro using multipotent O9-1 neural crest cells and polyacrylamide hydrogels of varying stiffness.