Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

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2.4K Views

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07:17 min

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August 2nd, 2021

DOI :

10.3791/62842-v

August 2nd, 2021

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Laura K. Hillert-Richter¹, Inna N. Lavrik¹

¹Translational Inflammation Research, Center of Dynamic Systems, Otto von Guericke University Magdeburg

Podsumowanie

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Protein A Sepharose Beads

Cell Suspension

Lysate

Antibody Incubation

Protein Concentration

Lysis Buffer

Flow Cytometry

Cold PBS

Rozdziały w tym wideo

0:05

Introduction

0:44

Preparing Cells and CD95L Stimulation

1:11

Cell Harvest and Lysis

2:29

Immunoprecipitation

3:28

Western Blot

4:21

Western Blot Detection

5:05

Analysis of CD95 DISC Formation in HeLa-CD95 cells, c-FLIPL-overexpressing HeLa-CD95 cells, and Primary T Cells

6:25

Conclusion

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