Analysis of CD95 DISC Formation in HeLa-CD95 cells, c-FLIPL-overexpressing HeLa-CD95 cells, and Primary T Cells
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Conclusion
필기록
Apoptosis initiation is mediated by activation of caspases at marker molecular platforms. We present the detection of the key initiation cell death complex DISC, which serves as a platform for caspase-8 activation. The advantage of this technique
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Here, an experimental workflow is presented that enables the detection of caspase-8 processing directly at the death-inducing signaling complex (DISC) and determines the composition of this complex. This methodology has broad applications, from unraveling the molecular mechanisms of cell death pathways to the dynamic modeling of apoptosis networks.