Methods Article

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

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9.0K Views

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08:56 min

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September 15th, 2021

DOI :

10.3791/63097-v

September 15th, 2021

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Yonatan R. Lewis-Israeli¹^,², Brett D. Volmert¹^,², Mitchell A. Gabalski¹^,², Amanda R. Huang¹^,², Aitor Aguirre¹^,²

¹Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, ²Department of Biomedical Engineering, College of Engineering, Michigan State University

Podsumowanie

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Human Heart Organoids

Pluripotent Stem Cells

3D Structure

Cardiovascular Diseases

Congenital Heart Defects

Cell Culture Protocol

Differentiation Steps

Embryoid Bodies

PSC Medium

Organoid Handling

High Throughput Method

Pharmacological Screening

Cell Suspension

Centrifuge Protocol

Tissue Engineering

Rozdziały w tym wideo

0:04

Introduction

0:59

Generation of 3D Self-Assembling Human Heart Organoids

2:51

Human Heart Organoid (hHO) Differentiation

5:33

Organoid Analysis

6:03

Results: Self-organizing hHO

8:02

Conclusion

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