Generation of 3D Self-Assembling Human Heart Organoids
2:51
Human Heart Organoid (hHO) Differentiation
5:33
Organoid Analysis
6:03
Results: Self-organizing hHO
8:02
Conclusion
副本
This protocol allows us to create a complex 3D structure that closely resembles the developing human heart in a simple cost effective way, that is reproducible across cell lines. It consists of a single protocol with three steps of differentiation
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Here, we describe a protocol to create developmentally relevant human heart organoids (hHOs) efficiently using human pluripotent stem cells by self-organization. The protocol relies on the sequential activation of developmental cues and produces highly complex, functionally relevant human heart tissues.