Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

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4.6K Views

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08:26 min

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January 13th, 2022

DOI :

10.3791/63213-v

January 13th, 2022

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Yusaku Hontani*¹, Najva Akbari*¹, Kristine E. Kolkman², Chunyan Wu¹^,³, Fei Xia¹^,⁴, Kibaek Choe¹, Grace Zhang Wang¹, Mariya Sokolova¹, Joseph R. Fetcho², Chris Xu¹

¹School of Applied and Engineering Physics, Cornell University, ²Department of Neurobiology and Behavior, Cornell University, ³College of Veterinary Medicine, Cornell University, ⁴Meinig School of Biomedical Engineering, Cornell University

Podsumowanie

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Deep tissue Microscopy

Three photon Fluorescence Microscopy

In Vivo Imaging

Mouse Brain

Zebrafish Brain

Neurological Diseases

Alzheimer s Disease

Autism

High spatial Resolution Imaging

Non collinear Optical Parametric Amplifier

Laser Settings

Petri Dish Preparation

Tricaine Solution

Pancuronium Bromide

Anesthetization Technique

Rozdziały w tym wideo

0:05

Introduction

1:04

Three-Photon Microscopy Setup

2:01

Fish Preparation for Three-Photon Imaging

3:52

Intravital Imaging in Fish

5:50

Intravital Imaging in Mouse

6:39

Results: Three-Photon Imaging in Intact Mouse and Zebrafish Brain with High Spatiotemporal Resolution

7:52

Conclusion

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