Methods Article

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

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2.6K Views

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09:00 min

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June 10th, 2022

DOI :

10.3791/63999-v

June 10th, 2022

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Thanh Nguyen¹, Sang Joon Ahn², Richard West³, Ibra S. Fancher¹

¹Department of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, ²Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, ³University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute, University of Delaware

Podsumowanie

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Vascular Endothelial Cells

Rozdziały w tym wideo

0:04

Introduction

0:51

Tissue Dissection and Cleaning

4:39

Digestion of Isolated Arteries for Isolation of Endothelial Cells

6:01

Staining of Endothelial Cells Prior to Flow Cytometry

7:06

Results: Identification of Endothelial Cells Following Arterial Tissue Digestion

8:15

Conclusion

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