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Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

1.8K Views

10:26 min

October 21st, 2022

DOI :

10.3791/64315-v

October 21st, 2022


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Mitochondrial Health

Rozdziały w tym wideo

0:04

Introduction

1:13

Preparation of Cell Suspension

3:24

Live-Dead Analysis of Tumor Spheroids

4:05

Disintegration and Cell Suspension of Tumor Spheroids

5:02

Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads

6:19

Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs

7:28

Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts

9:30

Conclusion

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