Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

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2.7K Views

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08:57 min

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November 11th, 2022

DOI :

10.3791/64626-v

November 11th, 2022

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Serena L. Cornelius¹^,², Megan M. Colonnetta²^,³^,⁴^,⁵, Katherine E. Lake¹^,², Clayton A. Smith¹^,², Yu-An Zhang¹^,², Evanthia T. Roussos-Torres⁶, Sangeetha M. Reddy¹^,², Elizabeth H. Chen²^,³^,⁴^,⁵, Isaac S. Chan¹^,²^,³^,⁴

¹Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, ²Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, ³Department of Molecular Biology, University of Texas Southwestern Medical Center, ⁴Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, ⁵Department of Cell Biology, University of Texas Southwestern Medical Center, ⁶Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

Podsumowanie

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Differential Centrifugation

Rozdziały w tym wideo

0:04

Introduction

0:55

Collecting, Digesting Tissue, and Differential Centrifugation

4:11

Embedding Organoids in BECM and Collagen

7:22

Results: Immunofluorescent Imaging of Embedded Organoids

8:11

Conclusion

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