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Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

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08:57 min

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November 11th, 2022

DOI :

10.3791/64626-v

November 11th, 2022

•

2,598 Views

Serena L. Cornelius¹^,², Megan M. Colonnetta²^,³^,⁴^,⁵, Katherine E. Lake¹^,², Clayton A. Smith¹^,², Yu-An Zhang¹^,², Evanthia T. Roussos-Torres⁶, Sangeetha M. Reddy¹^,², Elizabeth H. Chen²^,³^,⁴^,⁵, Isaac S. Chan¹^,²^,³^,⁴

¹Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, ²Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, ³Department of Molecular Biology, University of Texas Southwestern Medical Center, ⁴Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, ⁵Department of Cell Biology, University of Texas Southwestern Medical Center, ⁶Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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Keyword Extraction Epithelial Organoids

Differential Centrifugation

Benchtop Shaking Incubator

Basal Medium

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