Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy

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3.0K Views

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10:53 min

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December 1st, 2023

DOI :

10.3791/65306-v

December 1st, 2023

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Perla Pérez-Treviño¹, Bianca Nieblas¹^,², Selma Romina López-Vaquera¹, Noemí García¹^,²^,³

¹Experimental Medicine and Advanced Therapies, The Institute for Obesity Research, Tecnologico de Monterrey, ²Escuela de Medicina y Ciencias de la Salud, Tecnologico de Monterrey, ³Preclinical Research Unit, Tecnologico de Monterrey

Podsumowanie

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Mitochondrial Density

Mitochondrial Distribution

Live Skeletal Muscle Fibers

Confocal Microscopy

Mitochondrial Network Remodeling

Physiological Stress

Pathological Conditions

Image Processing

Tetramethylrhodamine Ethyl Ester

TMRE

Skeletal Muscle Alteration

Cellular Function

Intervention Effects

Mitochondrial Dynamics

Histological Analysis

Rozdziały w tym wideo

0:00

Introduction

1:40

Rat Gastrocnemius Muscle Fiber Bundle Dissection

2:42

Confocal Live Cell Imaging of Rat Skeletal Muscle Mitochondria

5:43

Mitochondrial Density Analysis

8:07

Mitochondrial Distribution Analysis Using Fast Fourier Transform

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