Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice

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1.5K Views

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08:49 min

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October 6th, 2023

DOI :

10.3791/65735-v

October 6th, 2023

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Christina B. Schroeter*¹, Antonia Henes*¹, Anna Vogelsang¹, Alexander M. Herrmann¹, Stefanie Lichtenberg¹, Derya Cengiz¹, Vera Dobelmann¹, Niklas Huntemann¹, Christopher Nelke¹, Susann Eichler², Philipp Albrecht¹, Sven G. Meuth*¹, Tobias Ruck¹

¹Department of Neurology, Medical Faculty, Heinrich Heine University Duesseldorf, ²Department of Neurology, Institute of Translational Neurology, University Hospital Muenster

Podsumowanie

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Central Nervous System

Autoimmune Encephalomyelitis

EAE Model

Cell Isolation Protocol

Neuroinflammation

Neurodegeneration

Multiple Sclerosis

CNS resident Cell Types

Microglia

Oligodendrocytes

Astrocytes

Biomolecular Mechanisms

Inflammatory Pathways

Clinical Scoring System

Rozdziały w tym wideo

0:00

Introduction

1:58

Preparation of CNS Tissue for Isolating Cells in EAE Mice

3:54

Dissociation of CNS Tissue for Isolating Cells in EAE Mice

5:25

Removal of Debris and Red Blood Cells from CNS Cell Suspension of EAE Mice

7:18

Isolation of CNS Cell Types Using Magnetic Beads in Naive and EAE Mice

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