The increasing instance of obesity and Type 2 Diabetes has become a serious health problem. Duodenal-jejunal bypass surgery, DJB, can improve glucose metabolism and ameliorate insulin resistance. And its metabolic benefits independent of weight loss are not fully understood.
Diet-induced obese diabetical mice were selected for DJB surgery to determine the feasibility and vertical structure of the surgery and to provide technicals for subsequent research. Mice were injected intraperitoneally with 1%pentobarbital sodium solution and buprenorphine. After anesthetizing the mice, the toe pinch reflex was checked, placed on the board.
And then the depilatory cream was applied to the abdomen of the mice. Disinfect the skin three times with iodophor and ethyl alcohol. Cover the animal with a sterile drape and make a two centimeter incision from the xiphoid to the abdomen.
Use an abdominal retractor to expose the abdominal cavity. The jejunal five centimeter distal to the ligament of Treitz was double ligated with six zero silk suture. Cut the jejunum in the middle of the two ligations.
Pull the proximal jejunal incision five centimeter along the bowel to the jejunum to create the jejunojejunostomy. The anastomotic works of the two bowels were aligned horizontally and they used a 10 zero silk suture for natural enteromosis. Fixed force ends of the bowels and cut the incision in this.
Suture the second layer of the posterior intestinal wall with a four simple continuous suture, fixed both ends of the bowel and suture the anterior wall. Sutures the fourth layer of the anterior wall with a simple continuous suture and suture the thick layer of the plasma muscle with a horizontal various. Pull the distal jejunal incision to the duodenal one centimeter below the panerus and make the duodenal jejunal stormy in the same method.
Double-neck the duodenal with a 606 suture, two millimeter from the distal end of the duodenal jejunal and enteromosis. Cut in the middle and suture the dump with a 10 zero silk suture. Rearrange and return the intestines to the abdominal cavity.
Raise the abdominal cavity with 13 centigrade saline. Suture the muscle and the skin separately with a six zero silk. Disinfect skin with iodophor.
After the operation, inject saline and penicillin to prevent dehydration and infection. Inject buprenorphine for postoperative analgesia. After surgery, placed mouse on the electric blanket to prevent hyperthermia until it was awake.
On the postoperative day, food and water was restricted. On the first postoperative day, give the mice sugar water drink, observe the health of the mice daily. We can see that the survival rate is gradually increasing, indicating that we need intensive surgical training to master the DJB technique.
Mouse had similar body weight and daily food intakes before and eight weeks after surgery. DJB mice did not show significant weight loss compared to SHAM control mice at eight weeks after surgery. Benefit intake was reduced because of dietary transition during the seven day period.
Forming DJB and SHAM control surgery and returned to the preoperative level three weeks later. By measuring the blood glucose, we can see that the blood glucose of the mice decreased significantly after the DJB surgery and the glucose tolerance was also significantly improved. I believe that through the previous introduction you have a physical understanding of DJB surgery and have a full understanding of its improvement of blood glucose.
We can master this technology within 18 minutes. The most important thing is to remember all the procedures and be proficient in this technology. For example, the suturing process should be strict.
In our procedure, we believe that preoperative preparation and postoperative care play an important role in the entire surgical process. It could have answered key questions about obesity and metabolism and provide a pathway for procedures in related fields. We hope this technology will help you.
