Epon Post Embedding Correlative Light and Electron Microscopy

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08:47 min

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January 12th, 2024

DOI :

10.3791/66141-v

January 12th, 2024

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Shuyuan Wang*¹, Haiyan Xiong*¹^,², Qiyuan Chang*¹^,³, Xudong Zhuang*⁴^,⁵^,⁶, Yaochen Wu¹^,³, Xinrui Wang⁴^,⁵^,⁶, Congxian Wu¹, Zhifei Fu¹^,⁷^,⁸

¹Public Technology Service Center, Fujian Medical University, ²The School of Pharmacy, Fujian Medical University, ³The School of Basic Medical Sciences, Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁵Medical Research Center, Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience, Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology, Fujian Medical University

Transkrypcja

Podsumowanie

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Correlative Light And Electron Microscopy

CLEM

Fluorescence Microscopy

Electron Microscopy

Epon Embedding

MEosEM

Ultrastructure Preservation

Rozdziały w tym wideo

0:00

Introduction

0:44

Ultrathin Sectioning for Epon Post‐Embedding Correlative Light and Electron Microscopy (CLEM)

1:33

Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy

4:07

Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post‐Embedding Correlative Light and Electron Microscopy

6:16

Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy

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