Epon Post Embedding Correlative Light and Electron Microscopy

Please note that all translations are automatically generated. Click here for the English version.

1.2K Views

•

08:47 min

•

January 12th, 2024

DOI :

10.3791/66141-v

January 12th, 2024

•

Shuyuan Wang*¹, Haiyan Xiong*¹^,², Qiyuan Chang*¹^,³, Xudong Zhuang*⁴^,⁵^,⁶, Yaochen Wu¹^,³, Xinrui Wang⁴^,⁵^,⁶, Congxian Wu¹, Zhifei Fu¹^,⁷^,⁸

¹Public Technology Service Center, Fujian Medical University, ²The School of Pharmacy, Fujian Medical University, ³The School of Basic Medical Sciences, Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁵Medical Research Center, Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience, Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology, Fujian Medical University

Podsumowanie

Przeglądaj więcej filmów

Correlative Light And Electron Microscopy

CLEM

Fluorescence Microscopy

Electron Microscopy

Epon Embedding

MEosEM

Ultrastructure Preservation

Rozdziały w tym wideo

0:00

Introduction

0:44

Ultrathin Sectioning for Epon Post‐Embedding Correlative Light and Electron Microscopy (CLEM)

1:33

Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy

4:07

Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post‐Embedding Correlative Light and Electron Microscopy

6:16

Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy

Powiązane Filmy

09:10

Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

19.1K Views

13:13

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

16.0K Views

17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

13.1K Views

12:54

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

13.8K Views

08:00

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

11.4K Views

10:03

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

9.3K Views

07:33

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

10.4K Views

08:59

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

3.6K Views

08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

4.3K Views

11:30

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

3.6K Views