Epon Post Embedding Correlative Light e Microscopia Eletrônica

Please note that all translations are AI generated. Click here for the English version.

884 Views

•

08:47 min

•

January 12th, 2024

DOI :

10.3791/66141-v

January 12th, 2024

•

Shuyuan Wang*¹, Haiyan Xiong*¹^,², Qiyuan Chang*¹^,³, Xudong Zhuang*⁴^,⁵^,⁶, Yaochen Wu¹^,³, Xinrui Wang⁴^,⁵^,⁶, Congxian Wu¹, Zhifei Fu¹^,⁷^,⁸

¹Public Technology Service Center, Fujian Medical University, ²The School of Pharmacy, Fujian Medical University, ³The School of Basic Medical Sciences, Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁵Medical Research Center, Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience, Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology, Fujian Medical University

Transcrição

Resumo

Explore mais vídeos

Este m s no JoVE

edi o 203

Capítulos neste vídeo

0:00

Introduction

0:44

Ultrathin Sectioning for Epon Post‐Embedding Correlative Light and Electron Microscopy (CLEM)

1:33

Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy

4:07

Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post‐Embedding Correlative Light and Electron Microscopy

6:16

Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy

Vídeos relacionados

09:10

Microscópio óptico e Eletrônica (CLEM) como uma ferramenta para visualizar moléculas microinjetados e seus eucarióticas Sub-celulares Metas

19.0K Views

17:08

In vivo Clonal Rastreamento de Stem hematopoiéticas e células progenitoras Marcado por cinco proteínas fluorescentes usando confocal e microscopia multifotônica

13.1K Views

12:54

In Vivo Rastreamento 4-Dimensional de tronco hematopoéticas e células progenitoras em adultos mouse na calota craniana de Medula Óssea

13.8K Views

08:00

Correlativo Confocal e Microscopia Eletrônica 3D de uma célula sensorial específica

11.4K Views

10:03

Investigando os prejudiciais efeitos de baixa pressão Plasma esterilização na sobrevivência de esporos de Bacillus subtilis usando Live celular microscopia

9.3K Views

07:33

Preparação de amostras biológicas por congelamento de alta pressão, realce de contraste assistida por microondas e resina mínima incorporação para a imagem latente de Volume

10.3K Views