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In Situ Immunohistochemical Detection of Immune Cell Markers in Cancer Tissues

January 26th 2024



Summary

Multiplex cyclic immunohistochemistry allows in situ detection of multiple markers simultaneously using repeated antigen-antibody incubation, image scanning, and image alignment and integration. Here, we present the operating protocol for identifying immune cell substrates with this technology in lung cancer and paired brain metastasis samples.

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