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University Medical Center Goettingen

9 ARTICLES PUBLISHED IN JoVE

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Medicine

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)
René Günther 1, Martin Suhr 1, Jan C. Koch 1, Mathias Bähr 1,2, Paul Lingor 1,2, Lars Tönges 1
1Dept. of Neurology, University Medicine Göttingen, 2DFG Research Center for the Molecular Physiology of the Brain (CMPB), Göttingen, Germany

A mouse model for amyotrophic lateral sclerosis (ALS) is examined clinically and behaviorally. As a prerequisite for an accompanying immunohistological analysis the preparation of the spinal cord is depicted in detail.

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Immunology and Infection

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Kerstin Menck 1, Daniel Behme 1, Mathias Pantke 1, Norbert Reiling 2, Claudia Binder 1, Tobias Pukrop 1, Florian Klemm 1
1Department of Hematology and Oncology, University Medical Center Göttingen, 2Microbial Interface Biology Group, Research Center Borstel

We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.

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Bioengineering

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
Eva Wagner *1,2,3, Sören Brandenburg *1,2, Tobias Kohl 1,2, Stephan E. Lehnart 1,2,3,4
1Heart Research Center Goettingen, 2Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, 3German Center for Cardiovascular Research (DZHK) partner site Goettingen, 4BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine

In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.

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Neuroscience

Optogenetic Stimulation of the Auditory Nerve
Victor H. Hernandez 1,2,5, Anna Gehrt 1,3, Zhizi Jing 3, Gerhard Hoch 1, Marcus Jeschke 1, Nicola Strenzke 3, Tobias Moser 1,2,4
1InnerEarLab, Department of Otolaryngology, University Medical Center Goettingen, 2Bernstein Focus for Neurotechnology, University of Goettingen, 3Auditory Systems Physiology Group, Department of Otolaryngology, University Medical Center Goettingen, 4Center for Nanoscale Microscopy and Molecular Physiology of the Brain, University of Goettingen, 5Department of Chemical, Electronic, and Biomedical Engineering, University of Guanajuato

Cochlear implants (CIs) enable hearing by direct electrical stimulation of the auditory nerve. However, poor frequency and intensity resolution limits the quality of hearing with CIs. Here we describe optogenetic stimulation of the auditory nerve in mice as an alternative strategy for auditory research and developing future CIs.

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Neuroscience

Optimized Management of Endovascular Treatment for Acute Ischemic Stroke
Katharina Schregel 1,2, Daniel Behme 1, Ioannis Tsogkas 1, Michael Knauth 1, Ilko Maier 3, André Karch 4, Rafael Mikolajczyk 4,5, Mathias Bähr 3, Jörn Schäper 6, José Hinz 6, Jan Liman 3, Marios-Nikos Psychogios 1
1Institute of Neuroradiology, University Medical Center Goettingen, 2Department of Radiology, Brigham and Women's Hospital and Harvard Medical School, 3Department of Neurology, University Medical Center Goettingen, 4Department of Epidemiology, Helmholtz Center for Infection Research, 5Institute of Medical Epidemiology, Biostatistics and Informatics, Martin-Luther-University Halle-Wittenberg, 6Department of Anesthesiology, University Medical Center Goettingen

The outcome of patients with acute ischemic stroke depends on swift restoration of cerebral blood flow. This protocol aims at optimizing the management of such patients by minimizing peri-procedural timings and rendering the time from hospital admission to reperfusion as short as possible.

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Cancer Research

Utilizing High Resolution Ultrasound to Monitor Tumor Onset and Growth in Genetically Engineered Pancreatic Cancer Models
Robert-Guenther Goetze 1, Soeren M. Buchholz 1, Shilpa Patil 1, Golo Petzold 1, Volker Ellenrieder 1, Elisabeth Hessmann 1, Albrecht Neesse 1
1Department Gastroenterology and Gastrointestinal Oncology, University Medical Center Goettingen

This article describes the utilization of high-resolution ultrasound in genetically engineered pancreatic cancer mice. The primary aim is to provide a detailed instruction for detection and evaluation of endogenous pancreatic tumors.

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Medicine

Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts
Laura Diaz-Maue *1,2, Janna Steinebach *1, Michael Schwaerzle 8,9, Stefan Luther 1,3,4,6, Patrick Ruther 8,9, Claudia Richter 1,5,6,7
1Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, 2Research Electronics Department, Max Planck Institute for Dynamics and Self-Organization, 3Department of Pharmacology and Toxicology, University Medical Center Goettingen, 4Institute for Nonlinear Dynamics, Georg-August-University Goettingen, 5Department of Cardiology and Pneumology, University Medical Center Goettingen, 6German Center for Cardiovascular Research, DZHK e.V., partner site Goettingen, 7Laboratory Animal Science Unit, German Primate Center Leibniz Institute for Primate Research, 8Department of Microsystems Engineering (IMTEK), University of Freiburg, 9Cluster of Excellence BrainLinks-BrainTools, University of Freiburg

This work reports a method for controlling the cardiac rhythm of intact murine hearts of transgenic channelrhodopsin-2 (ChR2) mice using local photostimulation with a micro-LED array and simultaneous optical mapping of epicardial membrane potential.

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Bioengineering

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
Gabriela L. Santos 1,2, Tim Meyer 1,2, Malte Tiburcy 1,2, Alisa DeGrave 1,2, Wolfram-Hubertus Zimmermann 1,2,3,4,5, Susanne Lutz 1,2
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)

Presented here is a protocol to generate engineered connective tissues for a parallel culture of 48 tissues in a multi-well plate with double poles, suitable for mechanistic studies, disease modeling, and screening applications. The protocol is compatible with fibroblasts from different organs and species and is exemplified here with human primary cardiac fibroblasts.

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Biochemistry

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
Andreas Andric 1, Eva Wagner 1, Anne Heberle 1, Max Holzknecht 1, Alexander K. H. Weiss 1
1Research Institute for Biomedical Aging Research, University of Innsbruck

This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.

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