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The Sanford Burnham Institute for Medical Research

5 ARTICLES PUBLISHED IN JoVE

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Biology

Detection of Protein Ubiquitination
Yeun Su Choo 1, Zhuohua Zhang 1
1Signal Transduction Program, The Sanford Burnham Institute for Medical Research

Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.

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Biology

Fluorescent Labeling of Drosophila Heart Structures
Nakissa N. Alayari 1,2, Georg Vogler 2, Ouarda Taghli-Lamallem 2, Karen Ocorr 2, Rolf Bodmer 2, Anthony Cammarato 1,2
1Biology Department, San Diego State University, 2Development and Aging Program, NASCR Center, The Sanford Burnham Institute for Medical Research

Here we describe a basic protocol for fluorescent labeling of different elements of heart tubes from larva and adult Drosophila melanogaster. These specimens are well-suited for imaging via fluorescent or confocal microscopy. This technique permits detailed structural analysis of the features of the hearts from a powerful model organism.

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Biology

Visualizing the Beating Heart in Drosophila
Georg Vogler 1, Karen Ocorr 1
1Development and Aging Program, The Sanford Burnham Institute for Medical Research

Technique required for visualizing the beating heart in larval and adult Drosophila are presented. Each life stage requires a different methodology.

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Biology

Semi-automated Optical Heartbeat Analysis of Small Hearts
Karen Ocorr 1, Martin Fink 2, Anthony Cammarato 1,3, Sanford I. Bernstein 3, Rolf Bodmer 1
1Development and Aging Program, The Sanford Burnham Institute for Medical Research, 2Cardiac Electrophysiology Group, Dept. of Physiology, Anatomy and Genetics, The Sanford Burnham Institute for Medical Research, 3Biology Department and Heart Institute, San Diego State University

We have developed a Semi-automated Optical Heartbeat Analysis method (SOHA) for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts. We demonstrate the application of our methodology to the analysis of heart function in fruit fly and embryonic mouse hearts.

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Genetics

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
Soda Balla Diop 1, Ryan T. Birse 1, Rolf Bodmer 1
1Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute

This is a high fat diet feeding protocol to induce obesity in Drosophila, a model for understanding fundamental molecular mechanisms implicated in lipotoxicity. It also provides a high throughput triacylglyceride assay for measuring fat accumulation in Drosophila and potentially other (insect) models under various dietary, environmental, genetic or physiological conditions.

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