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Detection of Protein Ubiquitination

DOI :

10.3791/1293-v

August 19th, 2009

August 19th, 2009

42,262 Views

1Signal Transduction Program, The Sanford Burnham Institute for Medical Research

Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.

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Protein Ubiquitination

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