Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

0:52

Introduction

1:27

Preparing for Dissection

2:02

Harvesting the E16 Rat Embryos

3:19

Isolating the Forebrains

4:25

Digesting and Triturating the Forebrain Tissue

6:09

Washing, Filtering, and Counting the Dissociated Cells

7:03

Culturing the Dissociated Cells to Promote Aggregation

7:54

Preparing the Coverslips and Washing the Aggregates

9:15

Seeding the Coverslips with the Aggregates

11:06

Representative Connections Formed Among Aggregates

11:29

Conclusion