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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR)

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235 Views

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04:02 min

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October 27th, 2023

DOI :

10.3791/200638-v

October 27th, 2023

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M. Reza Roushan¹, Chuchi Chen¹, Parisa Ahmadi¹, Valerie C. A. Ward¹

¹Department of Chemical Engineering, University of Waterloo

Transcrição

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Keywords Agrobacterium mediated Transformation

Chlorella Vulgaris

Colony PCR cPCR

Gene Integration

Log Phase Culture

Supplemented LB Broth

Agrobacterium Tumefaciens

Induction Medium

Selective Media

DNA Agarose Gel

Da série

Stable Gene Integration in Chlorella vulgaris Using Agrobacterium tumefaciens

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