optimization of lc-ms methods for the analysis of alkaloids in plant samples

Classification of Tropane Alkaloids from Plant Metabolites Using LC-MS/MS

Although fully synthetic molecules have become more prominent in drug discovery in recent decades, nearly two-thirds of all approved drugs from the last 39 years are natural products or natural-product-inspired,1,2&#160;underscoring the continued importance of natural products research. Alkaloids, certain nitrogen-containing natural products, are especially prized for their medicinal properties. Tropane alkaloids (TAs) containing the [3.2.1.]-bicyclic nitrogen-containing system, are produced mostly by plants in the Solanaceae (nightshade), Erythroxylaceae, and Convolvulaceae families. Examples include atropine, scopolamine, and cocaine; multiple semi-synthetic or synthetic tropanes are also used clinically3. TAs and their derivatives are used to treat many conditions3,4 and several of these drugs appear on the WHO&#39;s 2023 List of Essential Medicines5. Because of their potent activities, TAs are also used recreationally (as stimulants or deliriants) and can cause poisoning upon ingestion of plants (or preparations) that contain them6,7. TAs are undesirable in human and animal food8 and can taint teas, spices, grains, honey, and herbal supplements9,10.&#160;Because of both their medicinal promise and ability to poison, analytical methods that can aid in the discovery of new TAs (and identification of known TAs) are useful.
In tandem mass spectrometry (MS/MS), &#34;mass filters&#34; (e.g., quadrupoles, time-of-flight tubes) are coupled together physically (&#34;in-space&#34;), or an instrument employs additional &#34;in-time&#34; reaction/separation steps. In-space MS/MS uses different modes to select and fragment different ions at the different mass filters (e.g., the quadrupoles of a triple-quadrupole or QQQ instrument). These different modes can be used to determine which specific fragments are made by a given ion (product ion scan), which ions in a sample yield certain fragments (precursor ion scan or PrIS) or undergo losses of a characteristic mass (neutral loss scan or NLS), or which specific compounds possess which specific fragments (multiple reaction monitoring). MS/MS, therefore, provides fragments that are useful for proposing structures for new compounds or confirming an existing compound&#39;s presence. MS/MS is increasingly used in the drug discovery, natural products chemistry, and metabolomics fields11,12,&#160;and has been used to profile alkaloid-containing species (for phytochemical characterization or chemotaxonomic analysis) and to detect and quantify specific alkaloids in food or medicinal plants10,13,14,15,16.
Despite the many mass spectrometry techniques available, there are challenges in finding new alkaloids. In addition to finding a candidate organism to screen, a full structural confirmation of an alkaloid is an arduous process that may include many different analytical techniques. Additionally, researchers could isolate a compound that is already known, wasting labor, time, and resources. This is especially difficult for TAs, where hundreds, if not thousands of TAs, many of which are isomeric with one another, are reported. The process of &#34;identifying the knowns and distinguishing them from the unknowns&#34; is known as dereplication. Databases of the retention times (r.t.s) and mass fragments of different TAs and other compounds are published to aid with this process17,18.&#160;Nonetheless, dereplication is laborious; merely annotating (i.e., assigning putative structures to) the alkaloids in a sample&#39;s entire LC-MS/MS chromatogram is time-consuming. Recently, both molecular networking19,20 and manual dereplication18,21,22 have been used for benzylisoquinoline, monoterpene indole, and tropane alkaloids, and PrISs have been used for &#34;structural filtering&#34; of spectra to identify pyrrolizidine and solanine-type alkaloids23,24.&#160;There are no specific methods or workflows available for rapid LC-MS/MS-based dereplication of TA-containing samples, however, even though TAs possess common, easily-identifiable fragments (Figure 1). The method described here uses a combination of data-dependent (DD) product ion scans, PrISs, and NLSs to annotate and classify TA structures in plants based on both the distinct fragmentation patterns for mono-, di-, and trisubstituted tropanes (Figure 1A) and the losses of common ester groups found in these alkaloids (Figure 1B). The study organisms are several species in the nightshade genus Datura. A rich source of diverse TAs, Datura has been used&#160;throughout the world&#39;s history for medicinal and cultural purposes17- and is a challenging matrix to dereplicate because of its numerous, structurally similar TAs, providing us with appealing samples upon which to test our method.

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study

In the simplest form of our research, we are trying to discover new molecules in plants. Plants are chemically complex, and some of these chemicals may be medicinally useful. We're especially interested in finding new alkaloids, which are certain molecules that contain nitrogen.Recently, we have so many new different alkaloid instruments and techniques available, so people discover more and more new molecules every day. With these instruments, we can answer questions like what chemicals are present, when are they present, and what part of the plant can they be found? Mass spectrometry as an analytical technique has really improved over the past 50 years.Instruments are more powerful, less expensive, and have higher resolution. And these techniques allow us to not only identify more plant molecules like alkaloids but also be more sure in our identification. This method is fast and sensitive.We were able to collect a full set of data on nightshade plants containing many tropane alkaloids in 60 to 90 minutes, and assign proposed structures to even though low upon those alkaloids. We're very interested in developing tandem mass spectrometry-based filtering methods for other classes of alkaloids and using methods like these to study how plant chemistry differs between different species, different plant parts, and different growth stages.

Optimization of LC-MS Methods for the Analysis of Alkaloids in Plant Samples

To begin, use a pre-chilled spatula to add 100 milligrams of plant tissue powder into a tiered polypropylene microcentrifuge tube. Then immediately add one milliliter of 20%methanol to the tube. Place the capped tube on a rocking shaker for three hours at room temperature.Next, centrifuge the tube at 9, 464 G for 10 minutes, and collect the supernatant into an LC-MS autosampler vial. Set up an LC-MS instrument with an electrospray ionization source and a reverse phase HPLC column. For HPLC, use 0.1%formic acid in water as solvent A and 0.1%formic acid in acetonitrile as solvent B.Adjust the column oven temperature to 45 degrees Celsius and the flow rate to 0.5 milliliters per minute.Equilibrate the column with 99%A and 1%B. Set up a 30-minute gradient for solvent B concentration to increase from 1%to 50%over 26 minutes, then rapidly return to 1%B at 26.01 minutes and hold for four minutes. Configure the mass spectrometer's operating parameters.Set the interface voltage to four kilovolts, the nebulizing gas flow to three liters per minute, and the heating gas flow to 10 liters per minute. Adjust the desolvation line temperature to 250 degrees Celsius, heat block temperature to 400 degrees Celsius, and interface temperature to 300 degrees Celsius. Set drying gas flow to 10 liters per minute and collision-induced dissociation gas pressure to 17 kilopascals.Build an MS method in electrospray ionization source positive mode, include both Q3 and Q1 scans covering the mass range of 100 to 1, 000 daltons with the Q1 scan serving as a survey event with automatic isotope exclusion. Add a product ion scan link to the Q1 scan with a mass window of 50 to 1, 000 daltons, collision cell energy of minus 20 volts, and an event time of less than 0.2 seconds. Next, adjust the MS method to add positive mode precursor ion scans equal to the length of the LC method with collision cell energies of minus 20 volts and event times of 0.75 seconds.Ensure that in all cases, automatic isotope exclude or deisotoping functions are enabled. Set the master charge values for monosubstituted, disubstituted, and trisubstituted tropane alkaloid fragments. Next, to the MS-MS method, add positive mode neutral loss scans equal to the length of the LC method with collision cell energies of minus 20 volts and event times of 0.75 seconds.Ensure that in all cases, automatic isotopic exclude or deisotoping functions are enabled. Set up the neutral loss masses of interest for esters on tropane alkaloids for esters derived from tiglic acid, acetyl groups, and phenyllactic or tropic acid esters. Download the LC-MS method and create data files for the samples of interest.Once the HPLC column is equilibrated at 45 degrees Celsius, inject 10 to 20 microliters of sample.

optimization-lc-ms-methods-for-analysis-alkaloids-plant

Research

JoVE Journal

Chemistry

85 visualizações. Para começar, use uma espátula pré-resfriada para adicionar 100 miligramas de pó de tecido vegetal em um tubo de microcentrífuga de polipropileno em camadas. Em seguida, adicione imediatamente um mililitro de 20% de metanol ao tubo. Coloque o tubo tampado em um agitador por três horas em temperatura ambiente.Em seguida, centrifugue o tubo a 9.464 G por 10 minutos e colete o sobrenadante em um frasco para injetáveis de amostrador automático LC-M...