August 7th, 2010
•Microscopic analysis of γH2AX foci, which form following the phosphorylation of H2AX at Ser-139 in response to DNA double-strand breaks, has become an invaluable tool in radiation biology. Here we used an antibody to mono-methylated histone H3 at lysine 4 as an epigenetic marker of actively transcribing euchromatin, to evaluate the spatial distribution of radiation-induced γH2AX formation within the nucleus.
Tags
Vídeos Relacionados
Quantification of γH2AX Foci in Response to Ionising Radiation
The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados