Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

0:44

Introduction

1:24

Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding

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Sample Preparation for Initial Fluorescence Unfolding Curve

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Measurement of Fluorescence Spectra

5:45

Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding

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Representative Results for the Measurement of Membrane Protein Unfolding

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Conclusion